Hprt1tm14(Ple103-EGFP/cre)Ems
Targeted Allele Detail
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Symbol: |
Hprt1tm14(Ple103-EGFP/cre)Ems |
Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 14, Elizabeth M Simpson |
MGI ID: |
MGI:3835525 |
Synonyms: |
HAP1-EGFP/cre, Hprt1tm14(mEMS675)Ems, Hprt1tm14(Ple103-EGFPcre;mEMS675)Ems, Ple103 |
Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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Alliance: |
Hprt1tm14(Ple103-EGFP/cre)Ems page
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Mutant Cell Lines: |
mEMS675, mEMS676, mEMS689, mEMS1031 |
Germline Transmission: |
Earliest citation of germline transmission:
J:145689
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Parent Cell Line: |
E14TG2a (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Project Collection: |
Pleiades Promoter Project
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Allele Type: |
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Targeted (Recombinase, Reporter) |
Mutation: |
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Insertion
Vector Type: Not Applicable
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Hprt1tm14(Ple103-EGFP/cre)Ems expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Homolog in Mouse |
Note |
human |
HAP1 (9001) |
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Mutation details: The Ple103-EGFP/cre transgene (pEMS1083) was designed with the 877 bp Ple103 minipromoter (HAP1-A; derived from a segment of the basal promoter element of the human huntingtin-associated protein 1 (HAP1) gene) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtsb-m3 mutant locus on the X chromosome.
(J:144244, J:164356)
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Activity: |
![](https://www.informatics.jax.org/webshare/images/rightArrow.gif) Tissue activity of this recombinase allele
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Driver:
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HAP1
(human)
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View phenotypes and curated references for all genotypes (concatenated display).
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Germ line transmission of mutant cell line mEMS675 has been established.
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Original: |
J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009; |
All: |
3 reference(s) |
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