Hprt1<tm16(Ple167-EGFP/cre)Ems> Targeted Allele Detail MGI Mouse (MGI:3835530)

Hprt1^{tm16(Ple167-EGFP/cre)Ems}
Targeted Allele Detail

Summary

Symbol:	Hprt1^{tm16(Ple167-EGFP/cre)Ems}
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 16, Elizabeth M Simpson
MGI ID:	MGI:3835530
Synonyms:	Hprt1^{tm16(mEMS681)Ems}, Hprt1^{tm16(Ple167-EGFPcre;mEMS681)Ems}, NLS-EGFP/cre, Ple167, POGZ-A-EGFP/cre
Gene:	Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance:	Hprt1^{tm16(Ple167-EGFP/cre)Ems} page

Mutation
origin

Mutant Cell Lines:	mEMS668, mEMS670, mEMS672, mEMS681
Germline Transmission:	Earliest citation of germline transmission: J:145689
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd
Project Collection:	Pleiades Promoter Project

Mutation
description

Allele Type:

Targeted (Recombinase, Reporter)

Mutation:

Insertion Vector Type: Not Applicable

Hprt1^{tm16(Ple167-EGFP/cre)Ems} expression driven by 1 gene

Mutation details: The Ple167-EGFP/cre transgene (pEMS1091) was designed with the 1200 bp Ple167 minipromoter (POGZ-A; derived from a portion of the promoter of the POGZ gene from the Pogo transposable element with ZNF domain (POGZ) gene) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. (J:144244, J:164356)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	POGZ (human) Summary of all recombinase alleles driven by POGZ. Comparative matrix view of recombinase activities Alliance human POGZ gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS681 has been established.

References

Original:	J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:	4 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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