Gene Expression Data
Assay Details
Assay
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Reference:
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J:70800
Henion TR, et al., Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis. J Biol Chem. 2001 Aug 10;276(32):30261-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3835809
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Gene symbol:
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B3gnt5
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Gene name:
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UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
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Probe:
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Lc3 synthase cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 11.0
(more )
Note: This is a sagittal section.
Specimen
6A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a sagittal section.
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Specimen
6C:
embryonic day 11.0
(more )
Specimen
6C:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7A:
embryonic day 14.0
(more )
Note: This is a coronal section.
Specimen
7A:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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This is a coronal section.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: telencephalon |
Present |
Regionally restricted |
7A
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Widespread expression is detected throughout the rostral caudal extent of the telencephalon.
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TS22: telencephalon ventricular layer |
Strong |
Regionally restricted |
7A
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There is intense expression in the ventricular and subventricular zone, with the exception of the ventromedial wall of the caudal telencephalon.
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Specimen
7C:
embryonic day 17.0
(more )
Note: This is a sagittal section.
Specimen
7C:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
This is a sagittal section.
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Specimen
7E:
embryonic day 17.0
(more )
Note: This is a sagittal section.
Specimen
7E:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 17.0
|
Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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This is a sagittal section.
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Structure |
Level |
Pattern |
Image |
Note |
TS25: thalamus |
Strong |
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7E
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Significant expression is found in thalamic nuclei.
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TS25: cerebellum |
Strong |
Regionally restricted |
7E
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There is substantial expression in the precerebellar neuroepithelium and postmitotic neurons of the deep cerebell nuclei.
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Specimen
Not shown:
embryonic day 17.0
(more )
Specimen
Not shown:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS25: medulla oblongata |
Present |
Regionally restricted |
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Expression is present in the inferior olive.
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TS25: pons |
Present |
|
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Expression is present in pontine nuclei.
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Specimen
8A:
postnatal adult
(more )
Note: This is a sagittal section.
Specimen
8A:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal adult
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
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This is a sagittal section.
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Specimen
8B:
postnatal adult
(more )
Note: This is a sagittal section.
Specimen
8B:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a sagittal section.
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Specimen
9:
postnatal week 8-12
(more )
Specimen
9:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal week 8-12
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: spleen |
Present |
Regionally restricted |
9_Antisense
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Expression is present in the follicle region. Other staining indicates that the expressing cells are B, and not T, lymphocytes.
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