Antibody:	Anti-Sidekick
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The antiserum used for this assay is specific for sidekick proteins but cannot differentiate between Sdk1 and Sdk2. Sdk2 has also been annotated, see Assay (MGI:3836407).

Structure	Level	Pattern	Image	Note
TS20: ureteric tip	Present		5A
TS20: ureteric trunk	Present		5A
TS20: primitive collecting duct	Present		5A
TS20: mesenchyme of metanephros	Absent		5A

Structure	Level	Pattern	Image	Note
TS20: ureteric tip	Present	Regionally restricted	5B	Expression was localized to cell membranes.
TS20: ureteric trunk	Present	Regionally restricted	5B	Expression was localized to cell membranes.

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Weak		5D
TS23: ureteric trunk	Present	Regionally restricted	5D	Expression was detected in the medullary ureteric bud.
TS23: medullary collecting duct	Present		5D
TS23: maturing glomerular tuft	Absent		5D	Expression was not detected in developing glomeruli.

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Weak		5E
TS23: ureteric trunk	Present	Regionally restricted	5E	Expression was detected in the medullary ureteric bud.
TS23: medullary collecting duct	Present		5E
TS23: maturing glomerular tuft	Absent		5E	Expression was not detected in developing glomeruli.