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Cert1Gt(RRF047)Byg
Gene trapped Allele Detail
Summary
Symbol: Cert1Gt(RRF047)Byg
Name: ceramide transporter 1; gene trap RRF047, BayGenomics
MGI ID: MGI:3837565
Synonyms: Certgt
Gene: Cert1  Location: Chr13:96679243-96776675 bp, + strand  Genetic Position: Chr13, 50.58 cM
Alliance: Cert1Gt(RRF047)Byg page
Cardiac defects in Cert1Gt(RRF047)Byg/Cert1Gt(RRF047)Byg embryos

Show the 4 phenotype image(s) involving this allele.

Mutation
origin
Mutant Cell Line:  RRF047
Germline Transmission:  Earliest citation of germline transmission: J:146172
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Gene trapped (Null/knockout, Reporter)
Mutation:    Insertion of gene trap vector     Vector: pGT0Lxf     Vector Type: gene trap
 
Mutation detailsThe gene trap vector, pGT2Lxf, was inserted between exons 3 and 4 disrupting the pleckstrin homology domain and resulting in a truncated protein product. The exact site of insertion was 18394 nucleotides downstream of the 5' end of the third intron. RT-PCR analysis failed to detect full length transcript in homozygous embryos. Western blot analysis detected little or no protein expression in homozygous embryos. (J:146172)
Sequence Tags: 
Sequence tag details (1 tag)
Genome Context: 
Genome Browser view of this mutation
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 6 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cert1 Mutation:  28 strains or lines available
Notes
All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.
References
Original:  J:146172 Wang X, et al., Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol. 2009 Jan 12;184(1):143-58
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory