Cert1Gt(RRF047)Byg
Gene trapped Allele Detail
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| Symbol: |
Cert1Gt(RRF047)Byg |
| Name: |
ceramide transporter 1; gene trap RRF047, BayGenomics |
| MGI ID: |
MGI:3837565 |
| Synonyms: |
Certgt |
| Gene: |
Cert1 Location: Chr13:96679243-96776675 bp, + strand Genetic Position: Chr13, 50.58 cM
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| Alliance: |
Cert1Gt(RRF047)Byg page
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Cardiac defects in Cert1Gt(RRF047)Byg/Cert1Gt(RRF047)Byg embryos
Show the 4 phenotype image(s) involving this allele.
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| Mutant Cell Line: |
RRF047 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:146172
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| Parent Cell Line: |
Other (see notes) (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Gene trapped (Null/knockout, Reporter) |
| Mutation: |
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Insertion of gene trap vector
Vector: pGT0Lxf
Vector Type: gene trap
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Mutation details: The gene trap vector, pGT2Lxf, was inserted between exons 3 and 4 disrupting the pleckstrin homology domain and resulting in a truncated protein product. The exact site of insertion was 18394 nucleotides downstream of the 5' end of the third intron. RT-PCR analysis failed to detect full length transcript in homozygous embryos. Western blot analysis detected little or no protein expression in homozygous embryos.
(J:146172)
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| Sequence Tags: |
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Sequence tag details (1 tag)
| Tag ID |
GenBank ID |
Method |
Tag Location (trapped strand)* |
Select |
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| Genome Context: |
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Genome Browser view of this mutation
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Cert1 Mutation: |
28 strains or lines available
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All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.
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| Original: |
J:146172 Wang X, et al., Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol. 2009 Jan 12;184(1):143-58 |
| All: |
4 reference(s) |
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Analysis Tools
