Antibody:	TGFbeta2 antiserum
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to biotin

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Weak	Regionally restricted		Weak expression in chondrocytes in the ribs and vertebra.
TS20: vertebral cartilage condensation	Weak	Regionally restricted		Weak expression in chondrocytes in the ribs and vertebra.

Structure	Level	Pattern	Image	Note
TS21: skeleton	Present	Regionally restricted		Expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: skeleton	Present	Regionally restricted		Expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: skeleton	Present	Regionally restricted		Expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: skeleton	Present	Regionally restricted		Little expression in the periosteum. Expression in osteocytes of bone and maturing chondrocytes such as those of tracheal cartilage.

Structure	Level	Pattern	Image	Note
TS25: skeleton	Present	Regionally restricted	3E	Expression in the hypertrophic cartilage and osteogenic zone of bone. Predominantly in the extracellular matrix surrounding osteocytes.

Structure	Level	Pattern	Image	Note
TS25: skeleton	Present	Regionally restricted		High levels of expression in osteogenic bone and hypertrophic cartilage.

Structure	Level	Pattern	Image	Note
TS20: heart	Present

Structure	Level	Pattern	Image	Note
TS21: heart	Present

Structure	Level	Pattern	Image	Note
TS22: heart	Present

Structure	Level	Pattern	Image	Note
TS23: heart	Present

Structure	Level	Pattern	Image	Note
TS24: heart	Present

Structure	Level	Pattern	Image	Note
TS25: heart	Trace			Expression barely detectable above background.

Structure	Level	Pattern	Image	Note
TS25: tooth epithelium	Present	Regionally restricted	3H	Expression in odontoblasts and in the mesenchymal primordium of the pulp.
TS25: tooth mesenchyme	Present	Regionally restricted	3H	Expression in odontoblasts and in the mesenchymal primordium of the pulp.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted		Restricted pattern of expression in the basement membrane of the cuboidal epithelium of the metanephric tubules.

Structure	Level	Pattern	Image	Note
TS25: renal tubule	Weak	Regionally restricted		Decreased expression in the tubular epithelium.
TS25: mesenchyme of metanephros	Present	Regionally restricted		Expression in mesenchymal cells supporting the tubules.

Structure	Level	Pattern	Image	Note
TS21: renal tubule	Present	Regionally restricted	5A	Expression at the base of the epithelium in a subpopulation of tubules. Increased expression in the basement membrane of the cuboidal epithelium of the metanephric tubules.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Strong	Regionally restricted	5B	Expression is stronger than that seen at E12.5, and is in almost all tubules of the kidney cortex.

Structure	Level	Pattern	Image	Note
TS23: renal tubule	Present	Regionally restricted	5C	Expression in kidney tubule epithelium.
TS23: adrenal cortex	Strong		5C

Structure	Level	Pattern	Image	Note
TS23: renal tubule	Present	Regionally restricted	5D	Expression in the epithelium of kidney tubules as well as in supporting cells at the base of the epithelium.

Structure	Level	Pattern	Image	Note
TS23: adrenal cortex	Strong		5F , 5E
TS23: adrenal medulla	Absent		5E , 5F

Structure	Level	Pattern	Image	Note
TS21: gland	Weak	Regionally restricted		Only slight expression in the adrenal gland.

Structure	Level	Pattern	Image	Note
TS25: adrenal gland	Strong			Persistent high level of expression.

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Weak			Authors report slight expression in the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS23: intestine epithelium	Strong			Significantly stronger expression in the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present			Decreased expression compared with earlier stages.
TS25: intestine	Present	Regionally restricted		Moderate expression in the smooth muscle of the intestine.

Structure	Level	Pattern	Image	Note
TS25: esophagus	Present	Regionally restricted		Moderate expression in the smooth muscle of the esophagus; no expression in the stratified squamous epithelium.

Structure	Level	Pattern	Image	Note
TS25: stomach	Absent			No expression in the stratified squamous epithelium of the forestomach.

Structure	Level	Pattern	Image	Note
TS21: stomach	Present	Regionally restricted	7A	Expression in the submucosal region just basal to the epithelium of the stomach. Expression also in the basement membrane.

Structure	Level	Pattern	Image	Note
TS21: stomach	Present	Regionally restricted	7B	Expression in the submucosa and and in the basement membrane. Well demarcated boundary of expression just basal to the mucosal layer. No expression in the mucosal layer.
TS21: stomach mesenchyme	Absent		7B	Authors state there was no expression in surrounding mesenchymal layers.

Structure	Level	Pattern	Image	Note
TS23: intestine	Present	Regionally restricted	7C	Expression in the submucosa of the intestine. Well-demarcated boundary of expression just basal to the mucosal layer.
TS23: intestine epithelium	Present		7C

Structure	Level	Pattern	Image	Note
TS20: liver	Present	Regionally restricted		Expression in megakaryocytes.

Structure	Level	Pattern	Image	Note
TS21: liver	Absent			No expression in hepatocytes.

Structure	Level	Pattern	Image	Note
TS25: bronchiole	Present	Regionally restricted	8C	Expression just basal to the epithelium in the bronchiole.
TS25: lung vascular element	Present	Regionally restricted	8C	Authors report expression in small blood vessels.

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present	Regionally restricted	9C	Expression in the basement membrane just basal to the cochlear epithelium.

Structure	Level	Pattern	Image	Note
TS25: inner ear	Present

Structure	Level	Pattern	Image	Note
TS22: midbrain meninges	Present		10A

Structure	Level	Pattern	Image	Note
TS22: brain meninges	Present		10B	Authors report expression in a thin layer of meningeal cells surrounding the brain.

Structure	Level	Pattern	Image	Note
TS21: spinal cord meninges	Present	Regionally restricted	10C	Expression in meninges surrounding the dorsal spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	10D	Expression in the thin layered meninges. Only weak staining in neurons.

Structure	Level	Pattern	Image	Note
TS22: midbrain meninges	Present		10E

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	10F	Expression in glial tissue surrounding the neurons.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	10G	Expression in astrocytes in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS25: meninges	Strong
TS25: brain	Present	Regionally restricted		Expression in glia surrounding neurons in the brain.
TS25: spinal cord	Present	Regionally restricted		Expression in spinal cord astrocytes.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Weak			Low levels of expression in the epithelial layer of the skin.

Structure	Level	Pattern	Image	Note
TS23: dermis	Present		11C
TS23: epidermis	Present		11C
TS23: hair follicle	Present		11C

Structure	Level	Pattern	Image	Note
TS25: epidermis	Present		11D	Expression primarily in the epidermis.
TS25: dermis	Weak		11D	Low levels of expression in the dermis.
TS25: hair follicle	Present		11D