Gene Expression Data
Assay Details
Assay
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Reference:
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J:147809
Masuda T, et al., Laser capture microdissection and cDNA array analysis for identification of mouse KIAA/FLJ genes differentially expressed in the embryonic dorsal spinal cord. Brain Res. 2009 Jan 16;1249:61-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3844026
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Gene symbol:
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Fstl5
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Gene name:
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follistatin-like 5
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Probe:
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Fstl5 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
Table 1:
embryonic day 10.5
(more )
Specimen
Table 1:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Strong |
Regionally restricted |
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Expression was high in the dorsal spinal cord.
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Specimen
3D left:
embryonic day 10.5
(more )
Specimen
3D left:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
3D ISH
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Expression was detected in the dorsolateral edges of the spinal cord, especially in the dorsal root entry zone. Expression was also noted in the motor neurons.
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TS17: neural tube floor plate |
Present |
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3D ISH
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TS17: dorsal root ganglion |
Present |
Regionally restricted |
3D ISH
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Expression was detected in dorsal root ganglion neurons.
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Specimen
3D right:
embryonic day 10.5
(more )
Note: Double labeled: blue - Fstl5; brown - Isl1 (assay MGI:3844024) and Is12 (assay MGI:3844025).
Specimen
3D right:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Fstl5; brown - Isl1 (assay MGI:3844024) and Is12 (assay MGI:3844025).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Regionally restricted |
3D ISH Islet-1/2
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Expression was detected in the dorsolateral edges of the spinal cord, especially in the dorsal root entry zone. Expression was also noted in the motor neurons.
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TS17: neural tube floor plate |
Present |
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3D ISH Islet-1/2
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TS17: dorsal root ganglion |
Present |
Regionally restricted |
3D ISH Islet-1/2
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Expression was detected in dorsal root ganglion neurons.
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Specimen
4D left:
embryonic day 12.5
(more )
Specimen
4D left:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
4D ISH
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Expression was detected lateral to the ventricular zone.
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TS20: dorsal root ganglion |
Weak |
Regionally restricted |
4D ISH
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Expression was weak in the dorsal root ganglion neurons.
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Specimen
4D right:
embryonic day 12.5
(more )
Note: Double labeled: blue - Fstl5; brown - Isl1 (assay MGI:3844024) and Is12 (assay MGI:3844025).
Specimen
4D right:
(close )
Genetic Background:
|
ICR
|
Age:
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embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
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section
|
Embedding:
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Cryosection
|
Note:
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Double labeled: blue - Fstl5; brown - Isl1 (assay MGI:3844024) and Is12 (assay MGI:3844025).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
4D ISH Islet-1/2
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Expression was detected lateral to the ventricular zone.
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TS20: dorsal root ganglion |
Weak |
Regionally restricted |
4D ISH Islet-1/2
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Expression was weak in the dorsal root ganglion neurons.
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