Summary | Transgene origin | Transgene description | Expression | Recombinase activity | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Tg(Six2-EGFP/cre)1Amc
Name:	transgene insertion 1, Andrew P McMahon
MGI ID:	MGI:3845228
Synonyms:	Six2Cre, Six2-TGC(tg), Six2-TGCtg, Tet-off-eGFPCre BAC transgenic, TGC BAC transgenic allele, Tg(Six2-tTA,tetO-EGFP/cre)1Amc
Transgene:	Tg(Six2-EGFP/cre)1Amc  Location: unknown
Alliance:	Tg(Six2-EGFP/cre)1Amc page

Strain of Origin:

CD-1

Transgene Type:    Transgenic (Recombinase) Mutation:    Insertion   Tg(Six2-EGFP/cre)1Amc expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Six2 (MGI:102778)   Mutation details: The 181 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RPCI23-311C1, containing the entire Six2 locus (and other genes), was modified by targeting a Tet-off-eGFPCre into the ATG start site of the Six2 locus. This inserted a Tet-off cassette (tetracycline-regulated transactivator (tTA), 2xpolyA signal, tetracycline-responsive element (TRE or tetO with CMV min promoter) followed by an Enhanced Greed Fluorescent Protein/Cre Recombinase (EGFP/Cre) fusion protein coding sequence, (SV40 polyA signal), and frt-flanked kanamycin cassette. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into CD-1 zygotes. Sequence updates published after this transgene was created revealed an upstream Six2 ATG start site that is in-frame with the tTA; thus an apparent and unintended fusion was created with the tTA (80 amino acids potentially added) that renders transgene expression unaffected by tetracycline/doxycycline administration. (J:148455)

Activity:	Tissue activity of this recombinase allele
Driver:	Six2 (mouse) Summary of all recombinase alleles driven by Six2. Alliance mouse Six2 gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

398 assay results 17 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:148455 Kobayashi A, et al., Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development. Cell Stem Cell. 2008 Aug 7;3(2):169-81
All:	81 reference(s)