Gene Expression Data
Assay Details
Assay
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Reference:
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J:14429
Thomas T, et al., Genes coding for basement membrane glycoproteins laminin, nidogen, and collagen IV are differentially expressed in the nervous system and by epithelial, endothelial, and mesenchymal cells of the mouse embryo. Exp Cell Res. 1993 Sep;208(1):54-67
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3845870
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Gene symbol:
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Lamb1
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Gene name:
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laminin B1
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Probe:
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Lamb1 cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2B:
embryonic day 12.5
(more )
Specimen
2B:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Absent |
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2B
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No expression in neural tissue.
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TS20: embryo mesenchyme |
Present |
Regionally restricted |
2B
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Expression in mesenchyme cells surrounding the central nervous system destined to become the meninges.
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TS20: tail myocoele |
Strong |
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2B
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High levels of expression in myotome destined to form skeletal muscle.
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Specimen
2F:
embryonic day 12.5
(more )
Specimen
2F:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: blood vessel |
Weak |
Regionally restricted |
2F
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Expression in differentiating capillaries.
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Specimen
5B:
embryonic day 12.5
(more )
Specimen
5B:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B:
embryonic day 12.5
(more )
Specimen
6B:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: oral region |
Strong |
Regionally restricted |
6B
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High levels of expression in mesenchymal cells of the lower jaw and palate.
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TS20: tongue |
Weak |
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6B
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Localized to ectodermal cells forming the outer layer of the tongue.
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Specimen
7B:
embryonic day 12.5
(more )
Specimen
7B:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8B:
embryonic day 12.5
(more )
Specimen
8B:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: stomach epithelium |
Strong |
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8B
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Uniform high expression in epithelial cells and surrounding mesenchyme.
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TS20: stomach mesenchyme |
Strong |
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8B
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Uniform high expression in epithelial cells and surrounding mesenchyme.
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TS20: liver |
Weak |
Regionally restricted |
8B
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Lower levels of expression in groups of cells in the epithelium and througout the mesenchyme.
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Specimen
9B:
embryonic day 12.5
(more )
Specimen
9B:
(close )
Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: metanephros |
Present |
Uniform |
9B
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Uniform expression in both epithelial and mesenchymal compartments.
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TS20: dorsal aorta |
Present |
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9B
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Expression in the aorta, essentially the same as in surrounding mesenchyme.
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