Ascl2tm1.1Cle
Targeted Allele Detail
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| Symbol: |
Ascl2tm1.1Cle |
| Name: |
achaete-scute family bHLH transcription factor 2; targeted mutation 1.1 Hans Clevers |
| MGI ID: |
MGI:3846671 |
| Synonyms: |
Ascl2floxed |
| Gene: |
Ascl2 Location: Chr7:142520566-142523001 bp, - strand Genetic Position: Chr7, 88.08 cM
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| Alliance: |
Ascl2tm1.1Cle page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:148715
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| Parent Cell Line: |
IB10/E14IB10 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Conditional ready, No functional change) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A FRT-flanked neomycin cassette and a loxP site were placed in the non-coding exon 1 with an additional loxP site placed downstream of exon 2. The neomycin cassette was removed by crossing mice with a Flipase transgenic strain leaving the lone coding exon flanked by loxP sites. An Xho site is destroyed in the introduction of the FRT and loxP sites to exon 1. Restriction digests on cDNA from heterozygote placenta and intestinal extracts indicate that the gene is imprinted in placental tissue (the maternal allele is expressed) while there is no imprinting in intestinal stem cells (both alleles are expressed).
(J:148715)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Ascl2 Mutation: |
15 strains or lines available
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| Original: |
J:148715 van der Flier LG, et al., Transcription factor achaete scute-like 2 controls intestinal stem cell fate. Cell. 2009 Mar 6;136(5):903-12 |
| All: |
7 reference(s) |
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Analysis Tools
