Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol:	Lmx1btm3(cre/ERT2)Rjo
Name:	LIM homeobox transcription factor 1 beta; targeted mutation 3, Randy L Johnson
MGI ID:	MGI:3849051
Synonyms:	Lmx1bN246K-Cre, Lmx1bN246K-IREScreERT2, Lmx1bPM-IREScreERT2
Gene:	Lmx1b  Location: Chr2:33450977-33530620 bp, - strand  Genetic Position: Chr2, 22.48 cM
Alliance:	Lmx1btm3(cre/ERT2)Rjo page

Germline Transmission:	Earliest citation of germline transmission: J:149241
Parent Cell Line:	PC3 (ES Cell)
Strain of Origin:	129S4/SvJae-Tg(Prm-cre)70Og

Allele Type:    Targeted (Inducible, Null/knockout, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Single point mutation   Lmx1btm3(cre/ERT2)Rjo expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Lmx1b (MGI:1100513)   Mutation details: A C->A transition (changing the asparagine N, at position 246 to lysine, K) was introduced into exon 5 to produce a null allele of Lmx1b. Subsequently, an IREScreERT2 cassette, followed by a loxP-flanked PGK-neo, was inserted into the 3'UTR of the locus by homologous recombination in PC3 ES cells. Cre mediated recombination then removed the PGK-neo cassette upon transmission from male chimeric mice. The protamine-cre transgene contributed by the PC3 cells was separated from the knock-in allele by crossing germline male mutants with C57BL/6 mice. (J:149241)

Strategy for generating the Lmx1btm2(cre/ERT2)Rjo and Lmx1btm3(cre/ERT2)Rjo alleles

Activity:	Tissue activity of this recombinase allele
Driver:	Lmx1b (mouse) Summary of all recombinase alleles driven by Lmx1b. Alliance mouse Lmx1b gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

2 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Lmx1b Mutation:	16 strains or lines available

Original:	J:149241 Qiu Q, et al., Lmx1b-expressing cells in the mouse limb bud define a dorsal mesenchymal lineage compartment. Genesis. 2009 Apr;47(4):224-33
All:	4 reference(s)