Gene Expression Data
Assay Details
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Assay
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Reference:
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J:148804
Yu VW, et al., FIAT is co-expressed with its dimerization target ATF4 in early osteoblasts, but not in osteocytes. Gene Expr Patterns. 2009 Jun;9(5):335-40
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3850858
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Gene symbol:
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Atf4
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Gene name:
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activating transcription factor 4
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Antibody:
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anti-ATF4 (sc-22800)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The figure shows either a tibia or femur.
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Results
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Specimen
5A:
embryonic day 16.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: femur |
Present |
Regionally restricted |
5A
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Expression is in osteoblasts surrounding the newly formed trabecular bone at the mid-shaft of the diaphysis.
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| TS24: tibia |
Present |
Regionally restricted |
5A
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Expression is in osteoblasts surrounding the newly formed trabecular bone at the mid-shaft of the diaphysis.
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Specimen
5C:
postnatal day 2
(more )
Specimen
5C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: femur |
Present |
Regionally restricted |
5C
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Expression is in most of the osteoblasts and lining cells.
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| TS27: tibia |
Present |
Regionally restricted |
5C
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Expression is in most of the osteoblasts and lining cells.
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Specimen
5E:
postnatal day 2
(more )
Specimen
5E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: femur |
Present |
Regionally restricted |
5E
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Expression is in most of the osteoblasts and lining cells.
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| TS27: tibia |
Present |
Regionally restricted |
5E
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Expression is in most of the osteoblasts and lining cells.
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Specimen
5G:
postnatal week 4
(more )
Specimen
5G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: femur |
Present |
Regionally restricted |
5G
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Expression is in some osteoblasts and lining cells, absent in osteocytes.
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| TS28: tibia |
Present |
Regionally restricted |
5G
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Expression is in some osteoblasts and lining cells, absent in osteocytes.
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