Tg(Tnnt2-rtTA,tetO-cre)1Wtp
Transgene Detail
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| Symbol: |
Tg(Tnnt2-rtTA,tetO-cre)1Wtp |
| Name: |
transgene insertion 1, William T Pu |
| MGI ID: |
MGI:3851371 |
| Synonyms: |
ctTA-Cre, iTNT-cre, Tg(Tnnt2-rtTA,tetO-cre)t26Bz, TNT-icre |
| Transgene: |
Tg(Tnnt2-rtTA,tetO-cre)1Wtp Location: unknown
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| Alliance: |
Tg(Tnnt2-rtTA,tetO-cre)1Wtp page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase, Transactivator) |
| Inducer: |
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doxycycline/tetracycline |
| Mutation: |
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Insertion
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Tg(Tnnt2-rtTA,tetO-cre)1Wtp expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| rat |
Tnnt2 (24837) |
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Mutation details: In one construct (Tnnt2-rtTA), a 0.5-kb rat Tnnt2 promoter was inserted in front of a nuclear-localized (nls) rtTA cDNA followed by an elongation factor-1 (EF1) polyadenylation signal. The Tnnt2-rtTA fragment was flanked by mouse H19 insulator sequences to prevent ectopic transcription influences from random insertion sites. The second construct (tetO-cre) was generated by cloning the nls-cre sequence downstream of the tetO-CMV minimal promoter fragment. Purified constructs were coinjected into fertilized FVB/N oocytes. Seven double-transgenic founders contained both constructs and transmitted them in Mendelian fashion indicating that both constructs were inserted at the same site. One line demonstrated reproducible recombination of the R26R reporter after doxycycline treatment and was propagated and maintained.
(J:150452, J:157454)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Tnnt2
(rat)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:150452 Zhou B, et al., Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart. J Clin Invest. 2009 Jun;119(6):1462-76 |
| All: |
16 reference(s) |
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Analysis Tools
