Mcph1Gt(RRO608)Byg
Gene trapped Allele Detail
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| Symbol: |
Mcph1Gt(RRO608)Byg |
| Name: |
microcephaly, primary autosomal recessive 1; gene trap RRO608, BayGenomics |
| MGI ID: |
MGI:4332146 |
| Gene: |
Mcph1 Location: Chr8:18645147-18853205 bp, + strand Genetic Position: Chr8, 10.24 cM, cytoband A3
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| Alliance: |
Mcph1Gt(RRO608)Byg page
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| Mutant Cell Line: |
RRO608 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:157933
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| Parent Cell Line: |
Other (see notes) (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Gene trapped (Null/knockout, Reporter) |
| Mutation: |
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Insertion of gene trap vector
Vector: pGT2Lxf
Vector Type: gene trap
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Mutation details: The vector, containing a splice acceptor sequence upstream of a reporter gene beta-galactosidase and neomycin phosphotransferase, was inserted into intron 12. This results in a truncated protein lacking 97 amino acids in the C-terminus. Insertion of the vector does not disrupt Angpt2, which is located in intron 12 of Mcph1. The expression of wild type mRNA is significantly reduced in all tissues, and the reporter is very weakly expressed, undetectable by western blot or in situ hybridization.
(J:157933)
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| Sequence Tags: |
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Sequence tag details (1 tag)
| Tag ID |
GenBank ID |
Method |
Tag Location (trapped strand)* |
Select |
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| Genome Context: |
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Genome Browser view of this mutation
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Schematic of the Mcph1Gt(RRO608)Byg gene-trap allele |
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Mcph1 Mutation: |
58 strains or lines available
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All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.
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| Original: |
J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008; |
| All: |
5 reference(s) |
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Analysis Tools
