Herc2Gt(AR0530)Wtsi
Gene trapped Allele Detail
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| Symbol: |
Herc2Gt(AR0530)Wtsi |
| Name: |
HECT and RLD domain containing E3 ubiquitin protein ligase 2; gene trap AR0530, Wellcome Trust Sanger Institute |
| MGI ID: |
MGI:4346577 |
| Synonyms: |
Herc2530 |
| Gene: |
Herc2 Location: Chr7:55699944-55881548 bp, + strand Genetic Position: Chr7, 33.42 cM
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| Alliance: |
Herc2Gt(AR0530)Wtsi page
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| Mutant Cell Line: |
AR0530 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:252045
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| Parent Cell Line: |
E14TG2a.4 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Project Collection: |
Sanger Inst. Gene Trap Res.
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| Allele Type: |
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Gene trapped (Null/knockout) |
| Mutation: |
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Insertion of gene trap vector
Vector: pGT0lxr
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Mutation details: A gene trap vector inserted into intron 2. RT-PCR analysis of mRNA from various tissues indicated formation of a truncated mRNA fused to beta-galactosidase (beta-geo). Sequencing analysis showed fusion of beta-galactosidase after amino acid residue 24 of HERC2. Immunoblot analysis revealed that protein levels are decreased by ~50% in the brain and kidney of heterozygous mice. HERC2 activities including ubiquitin ligase and stimulation of p53 activity are reduced, as shown by increased levels of USP33 protein and lower levels of p21 mRNA in heterozygous mice.
(J:252045)
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| Sequence Tags: |
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Sequence tag details (1 tag)
| Tag ID |
GenBank ID |
Method |
Tag Location (trapped strand)* |
Select |
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| Genome Context: |
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Genome Browser view of this mutation
13 gene trap insertions have trapped this gene
View all gene trap sequence tags in this region
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Herc2 Mutation: |
215 strains or lines available
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| Original: |
J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008; |
| All: |
2 reference(s) |
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Analysis Tools
