Probe:	Pkd1 cDNA4
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Strong		5A	Expression is uniform.
TS9: embryo endoderm	Strong		5A	Expression is uniform.
TS9: embryo mesoderm	Strong		5A	Expression is uniform.

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Regionally restricted	5A	There is strong expression in the outer decidua, and little to no expression in the inner decidua.
TS28: placenta	Strong		5A
TS28: uterus	Ambiguous	Regionally restricted	5A	Expression is weak to absent along the mesometerium or inner uterine mucosa, immediately surrounding the embryo.

Structure	Level	Pattern	Image	Note
TS15: migrating neural crest	Present		6A	Expression is present in the migrating neural crest, between the neural tube and the myotome.
TS15: neural tube	Strong	Regionally restricted	6A	There is strong expression in the inner (ependymal) and external (marginal) layers of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: spinal ganglion	Present		6B
TS17: ascending aorta	Present	Regionally restricted	6B	Expression is present in the aortic arch.
TS17: 1st branchial arch mesenchyme	Strong		6B
TS17: neural tube	Present	Non-Uniform	6B	Expression is stronger in the ependymal layer, where mitotic activity is high, than in the internal (migrating) neuroepithelium of the mantle.
TS17: telencephalon	Present	Non-Uniform	6B	Expression is stronger in the ependymal layer, where mitotic activity is high, than in the internal (migrating) neuroepithelium of the mantle.
TS17: head	Strong	Regionally restricted	6B	Expression is present in the ectodermal mesenchyme of the developing skull, particularly in the region of the nasal process and the sinuses.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present

Structure	Level	Pattern	Image	Note
TS20: arch of aorta	Strong		6C top

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		6C bottom

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Strong
TS20: trigeminal ganglion	Strong

Structure	Level	Pattern	Image	Note
TS21: gut mesenchyme	Strong			Expression is present in the smooth muscle layer (muscularis) of the gastrointestinal tract.
TS21: vertebral cartilage condensation	Strong			There is a strong signal from cephalic to caudal regions.
TS21: rib cartilage condensation	Strong
TS21: diaphragm	Strong

Structure	Level	Pattern	Image	Note
TS22: Meckel's cartilage	Present
TS22: adrenal medulla	Strong
TS22: adrenal cortex	Ambiguous			Expression is weak to absent.

Structure	Level	Pattern	Image	Note
TS24: spinal ganglion	Present		6D
TS24: cricoid cartilage	Present		6D
TS24: cervical vertebra	Present	Regionally restricted	6D	Expression is confined to the prechondrocytes at the periphery.
TS24: rib	Present	Regionally restricted	6D	Expression is confined to the prechondrocytes at the periphery.

Structure	Level	Pattern	Image	Note
TS24: thoracic aorta	Strong		6E
TS24: thyroid cartilage	Strong		6E
TS24: rib	Present	Regionally restricted	6E	Expression is confined to the prechondrocytes at the periphery.
TS24: vertebra	Present	Regionally restricted	6E	Expression is confined to the prechondrocytes at the periphery.

Structure	Level	Pattern	Image	Note
TS24: hyoid	Strong			There is strong expression in the cartilage.
TS24: trachea cartilaginous ring	Strong

Structure	Level	Pattern	Image	Note
TS25: metanephros	Present		6F
TS25: ureter	Present		6F
TS25: adrenal medulla	Strong		6F
TS25: adrenal cortex	Ambiguous		6F	Expression is weak to absent.

Structure	Level	Pattern	Image	Note
TS25: brain ventricular layer	Present	Regionally restricted		Expression is present in a thin line at the junction of the ventricular walls.
TS25: central nervous system	Present			Within the neuroepithelium the signal is localized over individual neural cells.
TS25: hyoid	Strong			There is strong expression in the cartilage.
TS25: cricoid cartilage	Strong
TS25: thyroid cartilage	Strong
TS25: trachea cartilaginous ring	Strong

Structure	Level	Pattern	Image	Note
TS26: intestine mesenchyme	Present	Non-Uniform		Expression is present in the mucosa and lamina propria, although less intense than in the muscularis.

Structure	Level	Pattern	Image	Note
TS15: trunk sclerotome	Strong			There is a strong signal associated with the mesenchymal cells forming individual sclerotomes.

Structure	Level	Pattern	Image	Note
TS19: cervical vertebral cartilage condensation	Present

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present		7A dark field , 7A bright field	Expression is present in the early cartilage condensation of the sclerotome/vertebrae.
TS20: lung mesenchyme	Strong		7A bright field , 7A dark field	Expression is present in the pulmonary mesenchyme induced by the bronchial epithelium.
TS20: lung epithelium	Present		7A dark field , 7A bright field	Expression levels are much weaker than those seen in the condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Strong		7B dark field , 7B bright field	There is strong expression in the condensing metanephric blastema, with more intense signal immediately surrounding the ureteric buds.

Structure	Level	Pattern	Image	Note
TS22: salivary gland mesenchyme	Strong		7C bright field , 7C dark field	There is strong expression in the condensing salivary gland mesenchyme.
TS22: salivary gland epithelium	Present		7C dark field , 7C bright field	Expression levels are much weaker than those seen in the condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS25: hindlimb mesenchyme	Strong	Regionally restricted	7D dark field , 7D bright field	There is strong expression in the early cartilage condensation in the hindlimb.

Structure	Level	Pattern	Image	Note
TS26: dermis	Strong	Non-Uniform	7E dark field , 7E bright field	A particularly strong signal is seen in the dermis surrounding the developing hair follicles, especially the nasal region.
TS26: epidermis	Absent		7E dark field , 7E bright field

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Strong			There is strong expression in the maturing pulmonary epithelium.
TS26: lung	Present			Expression is present in the developing intraparenchymal vessel walls, with particularly intense signal in the outer adventitial layer.

Structure	Level	Pattern	Image	Note
TS25: nephrogenic zone	Strong	Regionally restricted	8A	Expression is present in the cortical nephrogenic zone.
TS25: renal medulla	Present	Regionally restricted	8A	Expression is weaker in the medullary interstitium than in the cortex.
TS25: medullary tubule	Present		8A
TS25: ureter	Strong	Regionally restricted	8A	The strongest expression is present in the urothelium.
TS25: renal pelvis	Present		8A
TS25: metanephros	Strong	Regionally restricted	8A	Strong expression is present in the walls of the larger renal vessels.

Structure	Level	Pattern	Image	Note
TS27: nephrogenic zone	Present	Non-Uniform	8B	Stronger expression is present in the nephrogenic zone, with lesser signal present in more mature inner cortex.
TS27: renal cortex	Present	Non-Uniform	8B	Stronger expression is present in the nephrogenic zone, with lesser signal present in more mature inner cortex.
TS27: glomerular tuft	Present		8B