Rhobtb3<tm1a(KOMP)Wtsi> Targeted Allele Detail MGI Mouse (MGI:4362349)

Rhobtb3^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

Symbol:	Rhobtb3^{tm1a(KOMP)Wtsi}
Name:	Rho-related BTB domain containing 3; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4362349
Gene:	Rhobtb3 Location: Chr13:76017656-76092044 bp, - strand Genetic Position: Chr13, 40.95 cM, cytoband C1
Alliance:	Rhobtb3^{tm1a(KOMP)Wtsi} page
IMPC:	Rhobtb3 gene page

Mutation
origin

Mutant Cell Lines:	EPD0042_1_F07, EPD0042_1_G09, EPD0067_2_C01, EPD0067_2_D02, EPD0067_2_F02, EPD0067_2_F03
Germline Transmission:	Earliest citation of germline transmission: J:175295
Parent Cell Line:	JM8.F6 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	KOMP-CSD

Mutation
description

Allele Type:		Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:		Insertion Vector: L1L2_gt0
		Mutation details: The L1L2_gt0 cassette was inserted at position 76087333 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 76088202. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:226884)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	255 assay results
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available Cell Lines: 1 line available
Carrying any Rhobtb3 Mutation:	35 strains or lines available

Notes

Cell line EPD0042_1_G09 was not successfully used to make chimeric mice. Germline transmission was accomplished. J:226884

References

Original:	J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:	7 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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