Spag17tm1a(KOMP)Wtsi
Targeted Allele Detail
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| Symbol: |
Spag17tm1a(KOMP)Wtsi |
| Name: |
sperm associated antigen 17; targeted mutation 1a, Wellcome Trust Sanger Institute |
| MGI ID: |
MGI:4364991 |
| Gene: |
Spag17 Location: Chr3:99792722-100050638 bp, + strand Genetic Position: Chr3, 43.25 cM, cytoband F3
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| Alliance: |
Spag17tm1a(KOMP)Wtsi page
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| IMPC: |
Spag17 gene page |
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| Mutant Cell Lines: |
EPD0234_6_C05, EPD0234_6_G08 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:188991
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| Parent Cell Line: |
JM8.N4 (ES Cell)
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| Strain of Origin: |
C57BL/6N
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| Project Collection: |
KOMP-CSD
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| Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
| Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 99889093 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 99889997. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:148605, J:173534)
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J:211752 used EPD0234_6_G08 ES cells.
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| Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
| All: |
4 reference(s) |
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Analysis Tools
