Id1tm2(cre/ERT2)Bene
Targeted Allele Detail
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| Symbol: |
Id1tm2(cre/ERT2)Bene |
| Name: |
inhibitor of DNA binding 1, HLH protein; targeted mutation 2, Robert Benezra |
| MGI ID: |
MGI:4366863 |
| Synonyms: |
Id1IRES-creERT2 |
| Gene: |
Id1 Location: Chr2:152578171-152579330 bp, + strand Genetic Position: Chr2, 75.41 cM
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| Alliance: |
Id1tm2(cre/ERT2)Bene page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:154023
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| Parent Cell Line: |
CY2.4 (ES Cell)
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| Strain of Origin: |
B6(Cg)-Tyrc-2J
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Id1tm2(cre/ERT2)Bene expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Id1 (MGI:96396) |
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Mutation details: An encephalomyocarditis virus internal ribosomal entry site (EMCV IRES) and a tamoxifen-inducible recombinase cre/ERT2 coding sequence were inserted after the Id1 gene coding sequence. An FRT-Neo-FRT cassette was inserted after the IRES-cre/ERT2 sequence and before the 3' untranslated region. The Id1 protein and creERT2 protein are translated from a bicistronic messenger RNA with the endogenous polyadenylation sequence. CY2.4 ES cells (albino C57BL/6 derivation) were used for targeting. Southern blot positive clones were injected into co-isogenic B6J blastocyts. Positive chimeras were bred to albino B6J females. Albino pups were genotyped by PCR, and positive pups were bred to B6 ACTB::FLPE mice (Artemis Pharamaceuticals) to excise the Neo cassette in vivo.
(J:154023)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Id1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Id1 Mutation: |
10 strains or lines available
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| Original: |
J:154023 Nam HS, et al., High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009 Nov 6;5(5):515-26 |
| All: |
2 reference(s) |
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Analysis Tools
