Tg(Pgk1-EGFP)1Medv
Transgene Detail
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| Symbol: |
Tg(Pgk1-EGFP)1Medv |
| Name: |
transgene insertion 1, Alexander Medvinsky |
| MGI ID: |
MGI:4397704 |
| Synonyms: |
sEGFP |
| Transgene: |
Tg(Pgk1-EGFP)1Medv Location: unknown
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| Alliance: |
Tg(Pgk1-EGFP)1Medv page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:135901
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| Parent Cell Line: |
E14TG2aIV (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: The transgenic construct has the Pgk1 promoter and the EGFP gene separated by a floxed STOP cassette containing a puromycin resistance selection marker, two poly A sites and a trasncriptional pause cassette (synthetic pA site and a transcriptional pause site). The linearized construct was electroporated into E14TG2a subclone IV ES cells. Positive clones were injected into day 3.5 C57BL/6 blastocysts and chimeras were bred to C57BL/6 mice to establish the transgenic line.
(J:135901)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:135901 Gilchrist DS, et al., Labeling of hematopoietic stem and progenitor cells in novel activatable EGFP reporter mice. Genesis. 2003 Jul;36(3):168-76 |
| All: |
4 reference(s) |
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Analysis Tools
