Muc1tm1.1(cre/ERT2)Lcm
Targeted Allele Detail
|
|
| Symbol: |
Muc1tm1.1(cre/ERT2)Lcm |
| Name: |
mucin 1, transmembrane; targeted mutation 1.1, Lewis Murtaugh |
| MGI ID: |
MGI:4399063 |
| Synonyms: |
Muc1IC2, Muc1IRES-CreERT2 |
| Gene: |
Muc1 Location: Chr3:89136364-89140688 bp, + strand Genetic Position: Chr3, 39.02 cM
|
| Alliance: |
Muc1tm1.1(cre/ERT2)Lcm page
|
|
|
|
| Allele Type: |
|
Targeted (Inducible, Recombinase) |
| Inducer: |
|
tamoxifen |
| Mutation: |
|
Insertion
|
| |
|
Muc1tm1.1(cre/ERT2)Lcm expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Muc1 (MGI:97231) |
|
|
| |
|
Mutation details: This allele was derived by crossing mice bearing Muctm1(cre/ERT2)Lcm to Gt(ROSA)26Sortm1(FLP1)Dym mice to delete the frt site-flanked neomycin resistance cassette in the germline. The present, neo-, allele contains, downstream of the termination codon of the endogenous gene, an internal ribosomal entry site (IRES) followed by DNA encoding Cre recombinase joined to ERT2 and a single frt site (IRES-CreERT2-FRT). ERT2 is a triply mutated (G400V, M543A, L544A) human estrogen receptor ligand-binding domain (LBD) that renders the fusion protein highly sensitive to activation by 4-hydroxytamoxifen, but unresponsive to the receptor's native ligands. The cre/ERT2 fusion protein is expressed under control of the endogenous promoter without disrupting expression of the endogenous gene. Tamoxifen-induced Cre activity has been demonstrated in acinar and ductal cells of the adult pancreas and in their embryonic precursors.
(J:159445)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Muc1
(mouse)
|
| |
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Muc1 Mutation: |
39 strains or lines available
|
|
| Original: |
J:159445 Kopinke D, et al., Exocrine-to-endocrine differentiation is detectable only prior to birth in the uninjured mouse pancreas. BMC Dev Biol. 2010;10:38 |
| All: |
2 reference(s) |
|
Analysis Tools
