P3h1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:153144 Vranka J, et al., Expression of prolyl 3-hydroxylase genes in embryonic and adult mouse tissues. Cell Struct Funct. 2009;34(2):97-104
Assay type:	RNA in situ
MGI Accession ID:	MGI:4418166
Gene symbol:	P3h1
Gene name:	prolyl 3-hydroxylase 1

Probe:	P3H1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 3A: embryonic day 11.5 (more

)

Structure	Level	Image	Note
TS19: forelimb bud	Present	3A
TS19: hindlimb bud	Present	3A
TS19: 1st branchial arch	Present	3A	Expression is present in the maxillary and mandibular components of the first embryonic arch.

Specimen 3D: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: tail	Present	Regionally restricted	3D	Expression is present in the distal tip of the tail.
TS20: vibrissa	Present		3D
TS20: forelimb	Present		3D
TS20: hindlimb	Present		3D

Specimen 3G: embryonic day 13.5 (more

)

Structure	Level	Image
TS21: pinna	Present	3G
TS21: forelimb	Present	3G
TS21: hindlimb	Present	3G

Specimen 4A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Regionally restricted	4A	Expression is present in prechondrogenic skeletal elements, such as the cartilage condensations in the vertebrae.

Specimen 4B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: arch of aorta	Strong		4B
TS20: Meckel's cartilage	Present		4B

Specimen 4C: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: cranium	Present		4C	Expression is present in prechondrogenic skeletal elements, such as the cranial primordia. This includes developing cartilage primordia within the eye.
TS20: eye	Absent		4C

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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