Tg(Hnf1b-cre/ERT2)1Jfer
Transgene Detail
|
|
| Symbol: |
Tg(Hnf1b-cre/ERT2)1Jfer |
| Name: |
transgene insertion 1, Jorge Ferrer |
| MGI ID: |
MGI:4420452 |
| Synonyms: |
Hnf1bCreER |
| Transgene: |
Tg(Hnf1b-cre/ERT2)1Jfer Location: unknown
|
| Alliance: |
Tg(Hnf1b-cre/ERT2)1Jfer page
|
|
| Strain of Origin: |
C57BL/6J x SJL
|
|
| Transgene Type: |
|
Transgenic (Inducible, Recombinase) |
| Inducer: |
|
tamoxifen |
| Mutation: |
|
Insertion
|
| |
|
Tg(Hnf1b-cre/ERT2)1Jfer expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Hnf1b (MGI:98505) |
|
|
| |
|
Mutation details: A BAC clone containing the Hnf1b locus (RP23-304H7) was modified by replacing exon 1 with a targeting vector containing an Hnf1b promoter fragment, cre/ERT2, IRES, d2EGFP (a destabilized variant of EGFP), the b-globin polyadenylation site, a FRT/neo/FRT cassette, and an intron 1 fragment that flanks exon 1 of Hnf1b. The targeting vector was electroporated into EL250 cells and homologous recombination was induced. Correctly targeted clones were treated to activate inducible Flp recombinase, removing the FRT/neo/FRT cassette. The recombined BAC was digested, linearized and microinjected into pronuclei of C57BL6/SJL eggs. 10 lines were generated, and after crossing to Rosa26 reporter mice, one line carrying a single copy of the transgene was maintained (after characterization of 4 lines exhibiting similar reporter recombination patterns.)
(J:156016)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Hnf1b
(mouse)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
| Original: |
J:156016 Solar M, et al., Pancreatic exocrine duct cells give rise to insulin-producing beta cells during embryogenesis but not after birth. Dev Cell. 2009 Dec;17(6):849-60 |
| All: |
17 reference(s) |
|
Analysis Tools
