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Gene Expression Data
Assay Details
Assay
Reference: J:74931 Tanaka M, et al., A mammalian oocyte-specific linker histone gene H1oo: homology with the genes for the oocyte-specific cleavage stage histone (cs-H1) of sea urchin and the B4/H1M histone of the frog. Development. 2001 Mar;128(5):655-64
Assay type: Northern blot
MGI Accession ID: MGI:4429441
Gene symbol: H1f8
Gene name: H1.8 linker histone
Probe: M5H8 cDNA
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Phosphorimaging
Assay notes: these are ovaries at different stages of the superovulation protocol.
Results
Image: 1B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 1.2 kb Amount Genetic BackgroundMutant Allele(s)Sex Note
CTRL P TS28: ovary Present 20 µg; total RNA C57BL/6J Female unstimulated ovaries
PMSG P TS28: ovary Present (a) 20 µg; total RNA C57BL/6J Female
3h P TS28: ovary Present 20 µg; total RNA C57BL/6J Female
6h P TS28: ovary Present 20 µg; total RNA C57BL/6J Female
9h P TS28: ovary Present 20 µg; total RNA C57BL/6J Female
12h P TS28: ovary Present 20 µg; total RNA C57BL/6J Female
24h P TS28: ovary Present 20 µg; total RNA C57BL/6J Female
48h P TS28: ovary Present 20 µg; total RNA C57BL/6J Female
Notes:
(a) The relative expression exhibited >50% reduction as early as 48 hours after the administration of PMSG when compared with the unstimulated state.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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