Epha4tm1.2Bzh
Targeted Allele Detail
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| Symbol: |
Epha4tm1.2Bzh |
| Name: |
Eph receptor A4; targeted mutation 1.2, Binhai Zheng |
| MGI ID: |
MGI:4430286 |
| Synonyms: |
EphA4mCFPNeoNull |
| Gene: |
Epha4 Location: Chr1:77343822-77491725 bp, - strand Genetic Position: Chr1, 39.55 cM, cytoband C1-C5
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| Alliance: |
Epha4tm1.2Bzh page
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Two loxP sites were inserted flanking exon 3. Preceding the floxed exon 3, a reporter/selection cassette (SA-mCFP-Neo) flanked by two Frt sites, containing a splice acceptor, a membrane-targeted cyan fluorescent protein reporter gene and a neo cassette, was inserted. After Cre-mediated excision of exon 3, a frameshift in the downstream coding sequence resulted in a null allele and a functional mCFP reporter. Western blot analysis on total brain extracts and immunostaining on adult brain tissue confirmed the absence of the protein in homozygous mutants. Immunohistochemistry also confirmed mCFP expression in the hippocampus.
(J:157159)
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Generation of the Epha4tm1Bzh, Epha4tm1.1Bzh and Epha4tm1.2Bzh alleles |
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Epha4 Mutation: |
67 strains or lines available
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| Original: |
J:157159 Herrmann JE, et al., Generation of an EphA4 conditional allele in mice. Genesis. 2010 Feb;48(2):101-5 |
| All: |
4 reference(s) |
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Analysis Tools
