Col1a1<tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae> Targeted Allele Detail MGI Mouse (MGI:4430599)

Col1a1^{tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae}
Name:	collagen, type I, alpha 1; targeted mutation 4, Rudolf Jaenisch
MGI ID:	MGI:4430599
Synonyms:	Col1a1 2lox 4F2A, Col1a1-2lox-4F2A, Col1a1^2lox-4F2A, Col1a1::2lox-tetO-4F2A, Col1a1^{2lox-tetO-4F2A}
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:157298
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:

Targeted (Inducible, Inserted expressed sequence, RMCE-ready)

Inducer:

doxycycline/tetracycline

Mutation:

Insertion

Col1a1^{tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae} expresses 4 genes

Mutation details: RMCE on KH2 cells inserted a cassette that contains a loxP-flanked pgkNEOpolyA cassette, a third loxP site, the tetracycline responsive element (TRE or tetO), the single polycistronic expression cassette 4F2A, SV40 intron plus polyA signal, and a fourth loxP site into the 3'UTR. The 4F2A cassette contains the four mouse reprogramming genes Oct4 (Pou5f1; POU domain, class 5, transcription factor 1), Sox2 (SRY-box containing gene 2), Klf4 (Kruppel-like factor 4 (gut)), and c-Myc (Myc; myelocytomatosis oncogene) separated by three different viral 2A oligopeptides that mediate ribosomal skipping (P2A [from porcine teschovirus-1], T2A [from insect Thosea asigna virus], and E2A [equine rhinitis A virus], respectively). (J:157298)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:157298 Carey BW, et al., Single-gene transgenic mouse strains for reprogramming adult somatic cells. Nat Methods. 2010 Jan;7(1):56-9
All:	7 reference(s)

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