Rnaseh2b<tm1a(EUCOMM)Wtsi> Targeted Allele Detail MGI Mouse (MGI:4432164)

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Rnaseh2b^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Summary

Symbol:	Rnaseh2b^{tm1a(EUCOMM)Wtsi}
Name:	ribonuclease H2, subunit B; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4432164
Synonyms:	Rnaseh2b^KOF, Rnaseh2b^tm1a, RNaseH2^null
Gene:	Rnaseh2b Location: Chr14:62569517-62610445 bp, + strand Genetic Position: Chr14, 33.19 cM, cytoband C3
Alliance:	Rnaseh2b^{tm1a(EUCOMM)Wtsi} page
IMPC:	Rnaseh2b gene page

Mutation
origin

Mutant Cell Lines:	EPD0087_4_A01, EPD0087_4_A02, EPD0087_4_A03, EPD0087_4_A04, EPD0087_4_B01, EPD0087_4_C01, EPD0087_4_C02, EPD0087_4_C04, EPD0087_4_D03, EPD0087_4_D04, EPD0087_4_E03, EPD0087_4_F03, EPD0087_4_G01, EPD0087_4_G03, EPD0087_4_H01, EPD0087_4_H02, EPD0087_4_H03, EPD0087_4_H04
Germline Transmission:	Earliest citation of germline transmission: J:186180
Parent Cell Line:	JM8.F6 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	EUCOMM

Mutation
description

Allele Type:		Targeted (Conditional ready, Hypomorph, Reporter)
Mutation:		Insertion Vector: L1L2_gt0
		Mutation details: The L1L2_gt0 cassette was inserted at position 62590506 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 62591370. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	7 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 1 line available
Carrying any Rnaseh2b Mutation:	43 strains or lines available

References

Original:	J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:	7 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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