Agl<tm1a(EUCOMM)Wtsi> Targeted Allele Detail MGI Mouse (MGI:4432988)

Agl^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

Symbol:	Agl^{tm1a(EUCOMM)Wtsi}
Name:	amylo-1,6-glucosidase, 4-alpha-glucanotransferase; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4432988
Gene:	Agl Location: Chr3:116533648-116601815 bp, - strand Genetic Position: Chr3, 50.47 cM
Alliance:	Agl^{tm1a(EUCOMM)Wtsi} page
IMPC:	Agl gene page

Mutation
origin

Mutant Cell Lines:	EPD0371_2_D09, EPD0371_2_D11, EPD0371_2_E12, EPD0371_2_H11
Germline Transmission:	Earliest citation of germline transmission: J:204812
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-A^tm1Brd
Project Collection:	EUCOMM

Mutation
description

Allele Type:		Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:		Insertion Vector: L1L2_Bact_P
		Mutation details: The L1L2_Bact_P cassette was inserted at position 116579900 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 116582802. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Expression

In Structures Affected by this Mutation:

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available Cell Lines: 1 line available
Carrying any Agl Mutation:	83 strains or lines available

References

Original:	J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:	7 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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