Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo
Transgene Detail
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| Symbol: |
Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo |
| Name: |
transgene insertion 1, Andrew Wolfe |
| MGI ID: |
MGI:4437221 |
| Synonyms: |
GnRH-CRETeR |
| Transgene: |
Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo Location: unknown
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| Alliance: |
Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo page
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| Strain of Origin: |
Not Specified
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| Transgene Type: |
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Transgenic (Inducible, Inserted expressed sequence, Recombinase) |
| Inducer: |
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doxycycline/tetracycline |
| Mutation: |
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Insertion
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Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Gnrh1 (MGI:95789) |
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Mutation details: A -3446 bp fragment of the mouse Gnrh1 promoter was fused upstream of cre recombinase. The proximal region of the Gnrh1 promoter was modified to insert two Tet operators (tetO2). In series, the transgene also contains the CMV promoter regulating the Tet repressor gene (tetR). The rabbit beta-globin intron II is inserted between the promoter and the gene to enhance expression of the tetR gene. In absence of doxycycline, the tet repressor protein binds to the tet operators, blocking transcription. Induction by doxycycline releases the repression, permitting transcription of cre recombinase. Four founders were generated and individually analyzed for function and specificity. A representative line no. 1 was created.
(J:157600)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Gnrh1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:157600 Wolfe A, et al., Temporal and spatial regulation of CRE recombinase expression in gonadotrophin-releasing hormone neurones in the mouse. J Neuroendocrinol. 2008 Jul;20(7):909-16 |
| All: |
1 reference(s) |
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Analysis Tools
