Mmut<tm1a(EUCOMM)Wtsi> Targeted Allele Detail MGI Mouse (MGI:4441659)

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Mmut^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Summary

Symbol:	Mmut^{tm1a(EUCOMM)Wtsi}
Name:	methylmalonyl-Coenzyme A mutase; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4441659
Gene:	Mmut Location: Chr17:41245576-41272879 bp, + strand Genetic Position: Chr17, 19.55 cM, cytoband C-D
IMPC:	Mmut gene page

Mutation
origin

Mutant Cell Lines:	EPD0509_2_A09, EPD0509_2_A10, EPD0509_2_A12, EPD0509_2_C09, EPD0509_2_C12, EPD0509_2_D09, EPD0509_2_D10, EPD0509_2_E09, EPD0509_2_E10, EPD0509_2_F09, EPD0509_2_G09, EPD0509_2_G12, EPD0509_2_H09
Germline Transmission:	Unknown
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-A^tm1Brd
Project Collection:	EUCOMM

Mutation
description

Allele Type:		Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:		Insertion Vector: L1L2_Bact_P
		Mutation details: The L1L2_Bact_P cassette was inserted at position 41248946 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 41250049. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 1 line available
Carrying any Mmut Mutation:	34 strains or lines available

References

Original:	J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:	2 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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