Sox18<tm1(GFP/cre/ERT2)Pzg> Targeted Allele Detail MGI Mouse (MGI:4442184)

Sox18^{tm1(GFP/cre/ERT2)Pzg}
Targeted Allele Detail

Summary

Symbol:	Sox18^{tm1(GFP/cre/ERT2)Pzg}
Name:	SRY (sex determining region Y)-box 18; targeted mutation 1, Pumin Zhang
MGI ID:	MGI:4442184
Synonyms:	Sox18-GCE, Sox18-GFP-Cre-ERT2, Sox18^{tm1(cre/ERT2)Zhang}
Gene:	Sox18 Location: Chr2:181311630-181313433 bp, - strand Genetic Position: Chr2, 103.71 cM
Alliance:	Sox18^{tm1(GFP/cre/ERT2)Pzg} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:159099
Parent Cell Line:	AB2.2 (ES Cell)
Strain of Origin:	129S7/SvEvBrd-Hprt1^b-m2

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase, Reporter)

Inducer:

tamoxifen

Mutation:

Insertion

Sox18^{tm1(GFP/cre/ERT2)Pzg} expression driven by 1 gene

Mutation details: Mice with this mutation have a tamoxifen-inducible Cre-mediated recombination system and, independently, express green fluorescent protein (GFP) from the promoter of the endogenous gene. A GFP-Cre/ERT2 cassette (GCE) with a polyadenylation signal followed by a frt site-flanked kanamycin and neomycin resistance cassette was inserted in the first targeting exon of the endogenous gene immediately following the ATG translation initiation codon. Although no green fluorescence is visible in whole heterozygous mutant E15.5 embryos or their urogenital tracts, immunohistochemical analysis detects GFP protein in kidneys and ovaries of these embryos. Following four-day tamoxifen induction of Cre recombinase activity in E15.5 embryos bearing this allele and a Cre recombinase-inducible lacZ reporter gene, beta-galactosidase activity is detected weakly by X-gal staining and beta-galactosidase protein more strongly by immunohistochemical analysis in the kidneys and ovaries. (J:159099)

Inheritance:

Not Specified

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sox18 (mouse) Summary of all recombinase alleles driven by Sox18. Alliance mouse Sox18 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Sox18 Mutation:	26 strains or lines available

References

Original:	J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010;
All:	6 reference(s)

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