Fzd2<tm1.1Nat> Targeted Allele Detail MGI Mouse (MGI:4442611)

Fzd2^tm1.1Nat
Targeted Allele Detail

Summary

Symbol:	Fzd2^tm1.1Nat
Name:	frizzled class receptor 2; targeted mutation 1.1, Jeremy Nathans
MGI ID:	MGI:4442611
Gene:	Fzd2 Location: Chr11:102495257-102498884 bp, + strand Genetic Position: Chr11, 66.45 cM
Alliance:	Fzd2^tm1.1Nat page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:159434
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation
description

Allele Type:		Targeted (Null/knockout, Reporter)
Mutations:		Insertion, Intragenic deletion
		Mutation details: The coding region for a nuclear-localized beta-galactosidase (nLacZ; using an SV40 nuclear localization signal) was targeted to the Fzd1 locus by homologous recombination. The nLacZ sequences replace the Fzd2 open reading frame. A LoxP-flanked neo selectable marker used for ES cell selection was removed by subsequent crossing to a mouse line expressing germline Cre recombinase. Sequences from the E coli tetracycline resistance gene (TetR) are included immediately 3' of the nLacZ sequences and are part of the 3' untranslated region of the nLacZ transcript. (J:159434)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background
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hm1	Fzd2^tm1.1Nat/Fzd2^tm1.1Nat	involves: 129S1/Sv * 129X1/SvJ * C57BL/6
hm2	Fzd2^tm1.1Nat/Fzd2^tm1.1Nat	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
cx3	Fzd2^tm1.1Nat/Fzd2^tm1.1Nat Fzd7^tm1.1Nat/Fzd7^tm1.1Nat	involves: 129 * C57BL/6
cx4	Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat	involves: 129 * C57BL/6
cx5	Dvl3^tm1Awb/Dvl3⁺ Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat	involves: 129 * C57BL/6
cx6	Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat Wnt3a^tm1Amc/Wnt3a⁺	involves: 129 * C57BL/6
cx7	Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat Wnt11^tm1Amc/Wnt11⁺	involves: 129 * C57BL/6
cx8	Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat Wnt5a^tm1Amc/Wnt5a⁺	involves: 129 * C57BL/6
cx9	Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat Vangl2^Lp/Vangl2⁺	involves: 129 * C57BL/6 * LPT/LeJ
cx10	Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat	involves: 129 * C57BL/6 * LPT/LeJ
cx11	Fzd2^tm1.1Nat/Fzd2⁺ Vangl2^Lp/Vangl2⁺	involves: 129 * C57BL/6 * LPT/LeJ
cx12	Fzd1^tm1.1Nat/Fzd1^tm1.1Nat Fzd2^tm1.1Nat/Fzd2^tm1.1Nat	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
cx13	Fzd1^tm1.1Nat/Fzd1^tm1.1Nat Fzd2^tm1.1Nat/Fzd2⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
cx14	Fzd1^tm1.1Nat/Fzd1⁺ Fzd2^tm1.1Nat/Fzd2^tm1.1Nat	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA
cx15	Fzd2^tm1.1Nat/Fzd2^tm1.1Nat Vangl2^Lp/Vangl2⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * LPT/LeJ
cx16	Fzd1^tm1.1Nat/Fzd1⁺ Fzd2^tm1.1Nat/Fzd2⁺ Vangl2^Lp/Vangl2⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * LPT/LeJ

Phenotypes:

Affected Systems	hm1	hm2	cx3	cx4	cx5	cx6	cx7	cx8	cx9	cx10	cx11	cx12	cx13	cx14	cx15	cx16
show or hide all annotated terms
cardiovascular system			√	√	√	√	√		√		√	√	√	√	√	√
decreased angiogenesis			√
disorganized yolk sac vascular plexus			√
abnormal heart morphology				√	√	√	√		√		√	√	√	√
abnormal heart development			√
double outlet right ventricle				√								√
overriding aortic valve												√
ventricular septal defect				√	√	√	√		√		√	√			√	√
craniofacial	√	√		N		√	√		√	√		√	√		√
craniofacial phenotype				N
short mandible												√
abnormal mouth morphology												√
abnormal palate development												√
palatal shelves fail to meet at midline												√
cleft palate	√	√				√	√		√	√		√	√		√
failure of palatal shelf elevation												√
digestive/alimentary system	√	√				√	√		√	√		√	√		√
abnormal palate development												√
palatal shelves fail to meet at midline												√
cleft palate	√	√				√	√		√	√		√	√		√
failure of palatal shelf elevation												√
embryo			√					√				N			√	√
embryo phenotype												N
disorganized yolk sac vascular plexus			√
incomplete embryo turning			√
embryonic growth retardation			√					√
decreased embryo size			√
abnormal embryonic tissue morphology			√
open neural tube			√												√	√
abnormal parietal yolk sac morphology			√
growth/size/body	√	√	√			√	√	√	√	√		√	√		√
abnormal mouth morphology												√
abnormal palate development												√
palatal shelves fail to meet at midline												√
cleft palate	√	√				√	√		√	√		√	√		√
failure of palatal shelf elevation												√
embryonic growth retardation			√					√
decreased embryo size			√
decreased body size		√
hearing/vestibular/ear												√			√
abnormal cochlear hair cell morphology												√			√
abnormal cochlear hair cell number												√			√
abnormal orientation of outer hair cell stereociliary bundles												√			√
homeostasis/metabolism												√
cyanosis												√
mortality/aging		√	√	√				√
neonatal lethality, incomplete penetrance		√
postnatal lethality, incomplete penetrance				√
embryonic lethality during organogenesis, complete penetrance			√					√
nervous system		√	√									√			√	√
nervous system phenotype		N
open neural tube			√												√	√
abnormal cochlear hair cell morphology												√			√
abnormal cochlear hair cell number												√			√
abnormal orientation of outer hair cell stereociliary bundles												√			√
abnormal nervous system electrophysiology		√
skeleton												√
short mandible												√
taste/olfaction		√
abnormal olfactory system physiology		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	6 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Fzd2 Mutation:	34 strains or lines available

References

Original:	J:159434 Nathans J, DDS for Fzd2^tm1.1Nat. MGI Direct Data Submission. 2010;
All:	5 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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