Ascl1<tm1.1(cre/ERT2)Jejo> Targeted Allele Detail MGI Mouse (MGI:4452601)

Ascl1^{tm1.1(cre/ERT2)Jejo}
Targeted Allele Detail

Summary

Symbol:	Ascl1^{tm1.1(cre/ERT2)Jejo}
Name:	achaete-scute family bHLH transcription factor 1; targeted mutation 1.1, Jane Johnson
MGI ID:	MGI:4452601
Synonyms:	Ascl1-CreER, Ascl1^CreERT2
Gene:	Ascl1 Location: Chr10:87326681-87329522 bp, - strand Genetic Position: Chr10, 43.64 cM
Alliance:	Ascl1^{tm1.1(cre/ERT2)Jejo} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:171439
Parent Cell Line:	W4 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutations:

Insertion, Intragenic deletion

Ascl1^{tm1.1(cre/ERT2)Jejo} expression driven by 1 gene

Mutation details: A targeting vector was designed to replace the entire coding region of the achaete-scute complex homolog 1 (Ascl1 or Mash1) with a CreERT2 fusion protein and a frt-flanked neomycin (neo) selection cassette. The construct was electroporated into 129S/SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were then bred to mice containing Flp-recombinase to remove the Frt-flanked sequence. These mice were then backcrossed at least 5 generations to and outbred ICR strain generate a colony of Ascl1-CreERT2 mice. (J:171439)

Generation of the Ascl1^{tm1.1(cre/ERT2)Jejo} allele

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ascl1 (mouse) Summary of all recombinase alleles driven by Ascl1. Comparative matrix view of recombinase activities Alliance mouse Ascl1 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Ascl1 Mutation:	28 strains or lines available

References

Original:	J:171439 Kim EJ, et al., Ascl1 (mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain. PLoS One. 2011;6(3):e18472
All:	75 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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