Carmil2tm1a(KOMP)Mbp
Targeted Allele Detail
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| Symbol: |
Carmil2tm1a(KOMP)Mbp |
| Name: |
capping protein regulator and myosin 1 linker 2; targeted mutation 1a, Mouse Biology Program, UC Davis |
| MGI ID: |
MGI:4462559 |
| Gene: |
Carmil2 Location: Chr8:106412906-106424819 bp, + strand Genetic Position: Chr8, 53.04 cM
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| Alliance: |
Carmil2tm1a(KOMP)Mbp page
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| IMPC: |
Carmil2 gene page |
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| Mutant Cell Lines: |
DEPD00524_2_B05, DEPD00524_2_C06, DEPD00524_2_D08, DEPD00524_2_G07 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:205441
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| Parent Cell Line: |
JM8A3.N1 (ES Cell)
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| Strain of Origin: |
C57BL/6N-Atm1Brd
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| Project Collection: |
KOMP-CSD
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| Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
| Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 106418328 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 106420373. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157064)
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View phenotypes and curated references for all genotypes (concatenated display).
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Cell line DEPD00524_2_G07 was successfully used to make chimeric mice. Germline transmission was accomplished. J:205441 |
| Original: |
J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010; |
| All: |
2 reference(s) |
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Analysis Tools
