Tg(Thy1-cre/ERT2,-EYFP)HGfng
Transgene Detail
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| Symbol: |
Tg(Thy1-cre/ERT2,-EYFP)HGfng |
| Name: |
transgene insertion H, Guoping Feng |
| MGI ID: |
MGI:4830789 |
| Synonyms: |
SLICK-H |
| Transgene: |
Tg(Thy1-cre/ERT2,-EYFP)HGfng Location: unknown
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| Alliance: |
Tg(Thy1-cre/ERT2,-EYFP)HGfng page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase, Reporter) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Thy1-cre/ERT2,-EYFP)HGfng expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Thy1 (MGI:98747) |
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Mutation details: The transgene was designed with a creERT2 fusion gene, two copies of the mouse Thy1 promoter, and an enhanced yellow fluorescent protein (EYFP) cDNA sequence. The creERT2 fusion gene (cre/ERT2) is cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand-binding domain. Each Thy1 promoter region is a modified regulatory region of the murine thy1.2 gene (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns). The two copies are placed "back to back" to function bidirectionally; one copy drives expression of CreERT2, the other copy drives expression of EYFP. Founder line H (SLICK-H) was subsequently established and backcrossed to C57BL/6J for 4 generations.
(J:134019)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Thy1
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:122791 Deisseroth K, et al., Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci. 2006 Oct 11;26(41):10380-6 |
| All: |
50 reference(s) |
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Analysis Tools
