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Gad1tm2(cre)Tama
Targeted Allele Detail
Summary
Symbol: Gad1tm2(cre)Tama
Name: glutamate decarboxylase 1; targeted mutation 2, Nobuaki Tamamaki
MGI ID: MGI:4833682
Synonyms: Gad1tm1(cre)Tama, GAD67-Cre
Gene: Gad1  Location: Chr2:70392109-70432358 bp, + strand  Genetic Position: Chr2, 41.63 cM, cytoband D
Alliance: Gad1tm2(cre)Tama page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:164349
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutations:    Insertion, Intragenic deletion
 
Gad1tm2(cre)Tama expression driven by 1 gene
 
Mutation details: A targeting vector was designed where the cre recombinase gene was inserted into immediately after the translational initiation site of the Gad1 (GAD67) gene in frame. This vector contained a 3-kb fragment at the 5' side, the cre gene inserted behind the Gad1 translational start, a Pgk-neo-p(A) cassette flanked by two Flp recognition target (FRT) sites, a 7-kb fragment at the 3' side, and an MC1 promoter-driven diphtheria toxin gene for selection. The vector was introduced into C57BL/6 ES cells and ES cells showing homologous recombination were injected into 8-cell stage CD-1 embryos. This allele is a null allele. (J:164349, J:173688)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Gad1 (mouse)
Summary of all recombinase alleles driven by Gad1.
 

Expression
In Mice Carrying this Mutation: 6 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gad1 Mutation:  65 strains or lines available
References
Original:  J:164349 Higo S, et al., Subtypes of GABAergic neurons project axons in the neocortex. Front Neuroanat. 2009;3:25
All:  12 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory