Tg(Car1-cre)5Flt
Transgene Detail
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| Symbol: |
Tg(Car1-cre)5Flt |
| Name: |
transgene insertion 5, James C Fleet |
| MGI ID: |
MGI:4835007 |
| Synonyms: |
CAC5 |
| Transgene: |
Tg(Car1-cre)5Flt Location: unknown
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| Alliance: |
Tg(Car1-cre)5Flt page
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| Transgene Type: |
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Transgenic (Recombinase) |
| Mutations: |
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Deletion, Insertion
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Tg(Car1-cre)5Flt expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Car1 (MGI:88268) |
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Mutation details: The 10.6 kb colon-specific promoter and the share 2.5 kb erythroid/colon enhancer from the mouse Car1 gene were PCR amplified and cloned in front of the cre recombinase gene in a plasmid vector. The purified construct was used to generated transgenic mice. Two lines were selected for further analysis. Line 5 (CAC5 with 40 copies to the transgene insertion) was shown by PCR to have 10-fold higher transgene mRNA levels than line 6 (CAC6 having 20 copies). The mRNA was detectable only in the large intestine from cecum to distal colon. A low level of mRNA expression was detected in the liver, but no other tissues analyzed expressed the transgene. In 2022, the investigator reported that ~3-22 copies of the transgene are integrated on chromosome 8 (85,597,602-85,597,668 [GRCm38]) and resulted in a 64bp deletion of genomic region in between the two identified breakpoints. There are no genes annotated in this region [NCBI genome browser - GRCm38 - Chr8:85,597,400-85,597,800].
(J:164247)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Car1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:164247 Xue Y, et al., Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestine. Mol Cancer Res. 2010 Aug;8(8):1095-104 |
| All: |
8 reference(s) |
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Analysis Tools
