Probe:	Frs2 alpha cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: embryo	Present	Ubiquitous	1A

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Widespread	1D	Expressed ubiquitously in most developing tissues.

Structure	Level	Pattern	Image	Note
TS19: 1st branchial arch	Present		1G
TS19: 2nd branchial arch	Present		1G
TS19: hindlimb bud	Present		1G
TS19: jaw primordium	Present		1G
TS19: liver parenchyma	Present		1G
TS19: midgut	Present		1G
TS19: tongue	Present		1G
TS19: vertebral pre-cartilage condensation	Present		1G
TS19: brain	Present		1G	Expression was detected in the neuroepithelium of the brain.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Strong		1I
TS22: lung	Strong		1I
TS22: rib cartilage condensation	Strong		1I
TS22: spinal cord	Strong		1I

Structure	Level	Pattern	Image	Note
TS22: comma-shaped body	Strong		1M , 1K
TS22: mesenchyme of metanephros	Strong		1M , 1K
TS22: renal tubule	Strong		1M , 1K
TS22: s-shaped body	Strong		1M , 1K
TS22: stomach epithelium	Strong		1M , 1K

Structure	Level	Pattern	Image	Note
TS22: brain	Strong		2A
TS22: midbrain ventricular layer	Strong	Regionally restricted	2A

Structure	Level	Pattern	Image	Note
TS22: brain	Strong		2C
TS22: telencephalon ventricular layer	Strong		2C
TS22: trigeminal ganglion	Strong		2C
TS22: cartilage	Present		2C

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present		2E	Expression was detected in the cortical plate and cortical subplate in the cingulate and parietal cortices.
TS26: cortical subplate	Present		2E	Expression was detected in the cortical plate and cortical subplate in the cingulate and parietal cortices.
TS26: caudate-putamen	Present		2E
TS26: telencephalon septum	Weak	Regionally restricted	2E	Expression was weak in the lateral septal nucleus.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present		2G	Expression was detected in the cortical plate and cortical subplate in the cingulate and parietal cortices.
TS26: cortical subplate	Present		2G	Expression was detected in the cortical plate and cortical subplate in the cingulate and parietal cortices.
TS26: caudate-putamen	Present		2G
TS26: telencephalon septum	Weak	Regionally restricted	2G	Expression was weak in the lateral septal nucleus.

Structure	Level	Pattern	Image	Note
TS26: hypothalamus	Weak		2I
TS26: thalamus	Weak		2I
TS26: dentate gyrus	Present		2I
TS26: hippocampus CA1	Present		2I
TS26: hippocampus CA2	Present		2I
TS26: hippocampus CA3	Present		2I