Probe:	Tcf7 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: forelimb bud presumptive apical ectodermal ridge	Moderate
TS15: limb mesenchyme	Strong			There is strong staining in the distal and proximal limb.
TS15: limb	Moderate			There is moderate staining in the anterior and posterior limb.
TS15: trunk somite	Strong
TS15: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS15: 1st branchial arch	Strong			Staining is present in the mandibular arch.
TS15: 2nd branchial arch	Strong			Staining is present in the hyoid arch.
TS15: eye	Moderate

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Strong
TS17: limb mesenchyme	Strong	Regionally restricted		Staining is present in the progress zone and middle limb.
TS17: limb	Moderate			There is moderate staining in the anterior and posterior limb.
TS17: trunk somite	Strong
TS17: 1st branchial arch maxillary component	Strong			Staining is present in the maxillary process.
TS17: 1st branchial arch	Strong			Staining is present in the mandibular arch.
TS17: 2nd branchial arch	Strong			Staining is present in the hyoid arch.
TS17: eye	Strong
TS17: nasal pit	Strong			Staining is present in the olfactory placode.
TS17: otocyst	Strong			Staining is present in the otic pit.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Moderate
TS19: limb mesenchyme	Strong	Non-Uniform		Staining is strong in the progress zone and moderate in the middle limb.
TS19: trunk somite	Moderate
TS19: maxillary process	Moderate
TS19: eye	Strong
TS19: genital tubercle	Strong			Staining is present in the external genitalia.
TS19: nasal pit	Strong			Staining is present in the olfactory placode.
TS19: inner ear	Strong			Staining is present in the otic pit.