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Evx2/Hoxd13tm5.1(Hoxd11/lacZ)Ddu
Targeted Allele Detail
Summary
Symbol: Evx2/Hoxd13tm5.1(Hoxd11/lacZ)Ddu
Name: even-skipped homeobox 2; targeted mutation 5.1, Denis Duboule
MGI ID: MGI:4850026
Synonyms: Evx2Hoxd13Tg(hoxd11lac)Ddu, HoxDTgHd11lacNsi, TgH13(d11lac), TgH[d-11/lac]Ge
Gene: Evx2  Location: Chr2:74483335-74489901 bp, - strand  Genetic Position: Chr2, 44.13 cM
Alliance: Evx2/Hoxd13tm5.1(Hoxd11/lacZ)Ddu page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:33879
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion
 
Mutation details: The E. coli lacZ gene was inserted, with translational reading frames aligned, into the first exon of the mouse Hoxd11 gene in a construct containing the entire Hoxd11 transcription unit and its known cis-acting 5'and 3' regulatory sequences. A construct containing this fusion gene followed by an oppositely oriented loxP-flanked PGK-neo selection cassette was targeted to the intergenic region between the transcription initiation sites of Evx2 and Hoxd13, at the 5' extremity of the Hoxd complex, with Hoxd11/lacZ in the same transcriptional orientation as Hoxd13. The selection cassette was subsequently deleted by Cre recombinase-mediated recombination, leaving a single loxP site downstream/3' of the Hoxd/lacZ fusion gene. (J:33879)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Evx2 Mutation:  19 strains or lines available
Notes
Cre recombinase-mediated recombination between the loxP site downstream of this insertion and a second loxP site elsewhere in the homologous chromosome may be used to delete the genes between the loxP sites, placing the gene following the deletion immediately downstream of Hoxd11 with its cis-acting regulatory elements.
References
Original:  J:33879 van der Hoeven F, et al., Gene transpositions in the HoxD complex reveal a hierarchy of regulatory controls. Cell. 1996 Jun 28;85(7):1025-35
All:  5 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory