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Myt1tm1(EGFP/icre)Ldh
Targeted Allele Detail
Summary
Symbol: Myt1tm1(EGFP/icre)Ldh
Name: myelin transcription factor 1; targeted mutation 1, Lynn D Hudson
MGI ID: MGI:4943749
Gene: Myt1  Location: Chr2:181405125-181469590 bp, + strand  Genetic Position: Chr2, 103.77 cM
Alliance: Myt1tm1(EGFP/icre)Ldh page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:169913
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S/SvEv
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Myt1tm1(EGFP/icre)Ldh expression driven by 1 gene
 
Mutation details: A targeting vector was designed to disrupt exon 2 with a fusion of EGFP and the modified Cre-recombinase, iCre , followed by a loxP site, and an frt-flanked neomycin (neo) resistance cassette. The vector also changed the initiation codon at the beginning of exon 2 to a GTG to ensure translation of the EGFP/iCre fusion protein. The neomycin cassette was removed by crossing mice with a transgenic strain expressing Tg(ACTFLPe)9205Dym leaving a single frt site and a loxP site downstream of the EGFP/iCre fusion. Northern blot confirmed the absence of mRNA. At E18, fluorescent cells are distributed throughout the spinal cord. (J:169913)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Myt1 (mouse)
Summary of all recombinase alleles driven by Myt1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 17 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Myt1 Mutation:  57 strains or lines available
References
Original:  J:169913 Hudson LD, et al., A tool for examining the role of the zinc finger myelin transcription factor 1 (Myt1) in neural development: Myt1 knock-in mice. Transgenic Res. 2011 Aug;20(4):951-61
All:  3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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11/12/2024
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