Tg(Hsd17b1-icre/ERT2)3Casa
Transgene Detail
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| Symbol: |
Tg(Hsd17b1-icre/ERT2)3Casa |
| Name: |
transgene insertion 3, Emilio Casanova |
| MGI ID: |
MGI:4946612 |
| Synonyms: |
Hsd17b1-iCreERT2 |
| Transgene: |
Tg(Hsd17b1-icre/ERT2)3Casa Location: unknown
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| Alliance: |
Tg(Hsd17b1-icre/ERT2)3Casa page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Hsd17b1-icre/ERT2)3Casa expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| Not Specified |
Hsd17b1 |
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Mutation details: A 200kb BAC containing the Hsd17b1 gene was modified by homologous recombination to generate the construct. The open reading frame sequence for codon-improved cre recombinase (icre) fused to a mutated ligand-binding domain (LBD) of the human estrogen receptor (ERT2) was inserted into the exon containing the transcription initiation codon of the Hsd17b1 gene. The initial targeting vector also contained the bovine growth hormone polyA signal and an Frt-flanked ampicillin cassette. The amp marker was subsequently removed from targeted by clones upon electroporation of a plasmid expressing Flp recombinase. Purified BAC clones were injected into (C57BL/6 x CBA)F1 oocytes. Three lines expressing the transgene were generated. Lines 1 and 2 were shown to contain 2 copies of the insert by Southern blot, while line 3 had 8 copies. Line 3 mRNA levels of icre/ERT2 were highest in ovaries and this line was used for subsequent analyses.
(J:170001)
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Generation of Tg(Hsd17b1-icre/ERT2)3Casa |
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Hsd17b1
(species not specified)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:170001 Grabner B, et al., A mouse tool for conditional mutagenesis in ovarian granulosa cells. Genesis. 2010 Oct 1;48(10):612-7 |
| All: |
2 reference(s) |
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Analysis Tools
