Probe:	Celsr3 cDNA1
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: brain	Present		1A	Expression was specifically in the brain, spinal cord and peripheral ganglia, and no signal was seen outside neural tissue.
TS17: future spinal cord	Present		1A	Expression was specifically in the brain, spinal cord and peripheral ganglia, and no signal was seen outside neural tissue.
TS17: peripheral nervous system	Present	Regionally restricted	1A	Expression was specifically in the brain, spinal cord and peripheral ganglia, and no signal was seen outside neural tissue.

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present	Non-Uniform	2A	Maximal intensity in post-migratory fields and lower intensity in ventricular zones.
TS20: cranial ganglion	Present		2A
TS20: retina	Present		2A
TS20: peripheral nervous system	Present	Regionally restricted	2A	Expression was detected in peripheral ganglia.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	2C , 2B	Expression was detected with maximal intensity in post-migratory fields and lower intensity in ventricular zones.
TS20: brain ventricular layer	Present	Regionally restricted	2C , 2B

Structure	Level	Pattern	Image	Note
TS22: nervous system	Present		3A
TS22: retina	Present		3A
TS22: diencephalon ventricular layer	Strong		3A
TS22: ganglionic eminence	Strong	Regionally restricted	3A	Expression was strong in the ventricular zone.
TS22: brain	Present	Regionally restricted	3A	In the lateral and median aspects of the hemispheres, migratory and post-migratory neuronal fields (intermediate zone, preplate, and early cortical plate) were more heavily labelled than periventricular zones.
TS22: cerebral cortex intermediate zone	Present		3A
TS22: cortical plate	Present		3A

Structure	Level	Pattern	Image	Note
TS25: brainstem	Present	Regionally restricted		Expression was present in the reticular formation and the cochleovestibular nuclei.
TS25: cerebellum	Present	Regionally restricted		Expression was present in the external granule layer.
TS25: diencephalon ventricular layer	Present
TS25: hypothalamus	Present
TS25: midbrain tegmentum	Present
TS25: midbrain ventricular layer	Present
TS25: tectum	Present
TS25: thalamus	Present	Regionally restricted		Expression was detected in the dorsal thalamus and the ventral thalamus.
TS25: retina	Present	Regionally restricted		Expression was detected in the ventricular zone and was high in the ganglion cells.
TS25: cortical plate	Present	Non-Uniform		Expression was detected in the cortical plate ventricular zone/subventricular zone and was high in the cortical plate proper.
TS25: corpus striatum	Present	Non-Uniform		Expression was detected in the striatum ventricular zone/subventricular zone and was high in the striatum.
TS25: main olfactory bulb	Present	Non-Uniform		Expression was high in the olfactory bulb. Expression was present in the mitral cells.

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	4A1	Expression was intense in the periventricular area.
TS25: cerebellum	Present	Regionally restricted	4A1	Expression was detected in the external granule layer.

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	4A2	Expression was intense in the periventricular area.

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present	Regionally restricted	4A3	Expression was intense in the periventricular area.

Structure	Level	Pattern	Image	Note
TS25: olfactory epithelium	Present		4A4
TS25: retina	Present		4A4
TS25: skin	Present		4A4

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Strong	Regionally restricted	5A	Expression was strong in the cortical plate.
TS27: cerebellum external granule cell layer	Strong		5A
TS27: corpus striatum	Strong		5A
TS27: dentate gyrus	Strong		5A
TS27: hippocampus pyramidal cell layer	Strong		5A
TS27: rostral migratory stream	Strong	Regionally restricted	5A	Expression was high in the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Strong	Regionally restricted	5B	Expression was strong in the cortical plate.
TS27: main olfactory bulb	Strong		5B

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong
TS28: rostral migratory stream	Strong	Regionally restricted		Expression was high in the rostral migratory stream.
TS28: diencephalon	Ambiguous	Regionally restricted		Expression was very low or undetectable in the ventricular zone.
TS28: dorsal thalamus	Ambiguous			Expression was very low or undetectable.
TS28: hypothalamus	Ambiguous			Expression was very low or undetectable.
TS28: prethalamus	Ambiguous			Expression was very low or undetectable.
TS28: cerebral cortex	Present	Regionally restricted		Expression was detected in the cortical plate proper but was very low or undetectable in the cortical plate ventricular zone/subventricular zone.
TS28: corpus striatum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the striatum and the ventricular zone/subventricular zone.
TS28: dentate gyrus	Present
TS28: hippocampus pyramidal cell layer	Present
TS28: main olfactory bulb granule cell layer	Present
TS28: main olfactory bulb mitral cell layer	Present

Structure	Level	Pattern	Image	Note
TS28: brain	Strong	Regionally restricted	6A	Expression was strong in periventricular zones.
TS28: cerebellum external granule cell layer	Strong		6A
TS28: neocortex	Strong	Regionally restricted	6A	Expression was strong in the superficial tier of the neocortical cortical plate.

Structure	Level	Pattern	Image	Note
TS28: brain	Strong	Regionally restricted	6B	Expression was strong in periventricular zones.
TS28: neocortex	Strong	Regionally restricted	6B	Expression was strong in the superficial tier of the neocortical cortical plate.
TS28: dentate gyrus	Strong		6B
TS28: hippocampus	Strong		6B

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong		6C
TS28: rostral migratory stream	Strong	Regionally restricted	6C	Expression was strong in the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Trace		7A
TS28: dentate gyrus	Trace		7A
TS28: rostral migratory stream	Trace	Regionally restricted	7A	Traces of expression were detected in the rostral migratory stream.