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Et(EGFP/cre)16250Rdav
Transgene Detail
Summary
Symbol: Et(EGFP/cre)16250Rdav
Name: enhancer trap 16250, Ron Davis
MGI ID: MGI:4947521
Transgene: Et(EGFP/cre)16250Rdav  Location: unknown  
Alliance: Et(EGFP/cre)16250Rdav page
Transgene
origin
Strain of Origin:  C57BL/6J
Project Collection: Neuroscience Blueprint cre
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
 
Et(EGFP/cre)16250Rdav expression driven by 1 gene
 
Mutation details: An enhancer trap lentiviral transgenic approach was used to generate these mice. A lentiviral vector transgene construct contained an EGFP/cre fusion gene under control of the mouse Thy1.2 minimal promoter (-270 to +30). The vector was designed with a self-inactivating lentiviral backbone (FUGW). The packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J embryos. Founder mice from line 16250 were crossed to B6-Gt(ROSA)26Sortm1Sor animals to assess cre activity/transmission. Cre positive mice were subsequently bred to C57BL/6 mice to generate a cre positive, reporter negative line. (J:150856)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Et(EGFP/cre)16250Rdav (mouse)
Summary of all recombinase alleles driven by Et(EGFP/cre)16250Rdav.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:150856 Davis R, Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University. MGI Direct Data Submission. 2009;
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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11/19/2024
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