Et(icre/ERT2)14624Rdav
Transgene Detail
|
|
| Symbol: |
Et(icre/ERT2)14624Rdav |
| Name: |
enhancer trap 14624, Ron Davis |
| MGI ID: |
MGI:4947573 |
| Transgene: |
Et(icre/ERT2)14624Rdav Location: unknown
|
| Alliance: |
Et(icre/ERT2)14624Rdav page
|
|
| Strain of Origin: |
FVB/N
|
| Project Collection: |
Neuroscience Blueprint cre
|
|
| Transgene Type: |
|
Transgenic (Inducible, Recombinase) |
| Inducer: |
|
tamoxifen |
| Mutation: |
|
Insertion
|
| |
|
Et(icre/ERT2)14624Rdav expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Et(icre/ERT2)14624Rdav (MGI:4947630) |
|
|
| |
|
Mutation details: An enhancer trap lentiviral transgenic approach was used to generate these mice. The transgene was designed with a self-inactivating lentiviral vector backbone (FUGW), a human synapsin minimal promoter (-244 to +47), a beta-globin intron, an icre /ERT2 fusion (icre; improved with mammalian codon usage, no putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), fused to a mutated human estrogen receptorligand binding domain (ERT2), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability. This packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J mouse embryos. Founders were crossed to C57BL/6 animals. Animals were crossed to B6-Gt(ROSA)26Sortm1Sor mice to assess cre activity/transmission. Cre positive, lacZ negative mice were subsequently bred to C57BL/6 mice to create the 14624 line.
(J:150856)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Et(icre/ERT2)14624Rdav
(mouse)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
| Original: |
J:150856 Davis R, Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University. MGI Direct Data Submission. 2009; |
| All: |
1 reference(s) |
|
Analysis Tools
