Col1a1<tm1(tetO-GFP/RNAi:luc)Slowe> Targeted Allele Detail MGI Mouse (MGI:4950574)

Col1a1^{tm1(tetO-GFP/RNAi:luc)Slowe}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm1(tetO-GFP/RNAi:luc)Slowe}
Name:	collagen, type I, alpha 1; targeted mutation 1, Scott Lowe
MGI ID:	MGI:4950574
Synonyms:	Col1a1^{tm1(TRE-GFP-shLuci.1309)Swl}, TG-luc.1309
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm1(tetO-GFP/RNAi:luc)Slowe} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:171191
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:		Targeted (Inducible, Knockdown, Reporter)
Inducer:		doxycycline/tetracycline
Mutation:		Insertion
		Mutation details: RMCE on KH2 cells inserted a cassette that contains the tetracycline promoter and a GFP fusion with an Mir30e-modified to contain a short hairpin sequence targeting luciferase (shLuc.1309) into the 3'UTR. (J:171191)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:171191 Premsrirut PK, et al., A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell. 2011 Apr 1;145(1):145-58
All:	8 reference(s)

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