Notch1<tm5(cre/ERT2)Rko> Targeted Allele Detail MGI Mouse (MGI:5009237)

Notch1^{tm5(cre/ERT2)Rko}
Targeted Allele Detail

Summary

Symbol:	Notch1^{tm5(cre/ERT2)Rko}
Name:	notch 1; targeted mutation 5, Raphael Kopan
MGI ID:	MGI:5009237
Synonyms:	N1::CreERT2, NIP1::CreER^T2, Notch1::CreERT2
Gene:	Notch1 Location: Chr2:26347914-26393834 bp, - strand Genetic Position: Chr2, 18.91 cM
Alliance:	Notch1^{tm5(cre/ERT2)Rko} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:220446
Parent Cell Line:	B6/BLU (ES Cell)
Strain of Origin:	C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutations:

Insertion, Intragenic deletion

Notch1^{tm5(cre/ERT2)Rko} expression driven by 1 gene

Mutation details: The targeting construct was designed to replace exons 29-34 (encoding the intracellular domain) with a CreER^T2 sequence and nuclear localization signal, followed immediately downstream by a C-terminal 6xMyc epitope tag, 2xSV40 polyadenylation sequence, and frt-flanked Pgk1/EM7 promoter-driven neomycin selection cassette. The CreER fusion gene (Cre-ER^T2) is Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain. (J:172930)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Notch1 (mouse) Summary of all recombinase alleles driven by Notch1. Comparative matrix view of recombinase activities Alliance mouse Notch1 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Notch1 Mutation:	117 strains or lines available

References

Original:	J:172930 Liu Z, et al., Rapid identification of homologous recombinants and determination of gene copy number with reference/query pyrosequencing (RQPS). Genome Res. 2009 Nov;19(11):2081-9
All:	7 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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