Cck<tm2.1(cre/ERT2)Zjh> Targeted Allele Detail MGI Mouse (MGI:5014094)

Cck^{tm2.1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

Symbol:	Cck^{tm2.1(cre/ERT2)Zjh}
Name:	cholecystokinin; targeted mutation 2.1, Z Josh Huang
MGI ID:	MGI:5014094
Synonyms:	Cck-CreER
Gene:	Cck Location: Chr9:121318890-121324728 bp, - strand Genetic Position: Chr9, 72.43 cM, cytoband F4
Alliance:	Cck^{tm2.1(cre/ERT2)Zjh} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:151755
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	C57BL/6
Project Collection:	Neuroscience Blueprint cre

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutation:

Insertion

Cck^{tm2.1(cre/ERT2)Zjh} expression driven by 1 gene

Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette. These Cck-CreER (or Cck-CreERT2) mice were subsequently bred to C57BL/6 inbred mice for two additional generations and then bred together for many more generations (and the FLPe transgene was removed). (J:151755)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cck (mouse) Summary of all recombinase alleles driven by Cck. Comparative matrix view of recombinase activities Alliance mouse Cck gene page

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Cck Mutation:	26 strains or lines available

References

Original:	J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009;
All:	9 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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