About   Help   FAQ
Otoftm1.1(cre)Mull
Targeted Allele Detail
Summary
Symbol: Otoftm1.1(cre)Mull
Name: otoferlin; targeted mutation 1.1, Ulrich Mueller
MGI ID: MGI:5014098
Synonyms: Otof-Cre
Gene: Otof  Location: Chr5:30524406-30619276 bp, - strand  Genetic Position: Chr5, 16.48 cM, cytoband B1
Alliance: Otoftm1.1(cre)Mull page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:151755
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Project Collection: Neuroscience Blueprint cre
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Otoftm1.1(cre)Mull expression driven by 1 gene
 
Mutation details: A targeting vector was designed to insert a nuclear-localized Cre recombinase gene and polyA signal followed by an FRT-flanked PGK-Neo cassette into the initiation codon of the otoferlin (Otof) locus. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice to establish the colony. Next, mutant mice were bred with FLPe-expressing mice on a C57BL/6 congenic background to remove the FRT-flanked PGK-Neo cassette. The resulting Otof-Cre mice were then bred to C57BL/6J inbred mice for approximately two generations (selecting away from the FLPe transgene). (J:151755)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Otof (mouse)
Summary of all recombinase alleles driven by Otof.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Otof Mutation:  110 strains or lines available
References
Original:  J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009;
All:  8 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory