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Otoftm1.1(cre)Mull
Targeted Allele Detail
Summary
Symbol: Otoftm1.1(cre)Mull
Name: otoferlin; targeted mutation 1.1, Ulrich Mueller
MGI ID: MGI:5014098
Synonyms: Otof-Cre
Gene: Otof  Location: Chr5:30524406-30619276 bp, - strand  Genetic Position: Chr5, 16.48 cM, cytoband B1
Alliance: Otoftm1.1(cre)Mull page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:151755
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Project Collection: Neuroscience Blueprint cre
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Otoftm1.1(cre)Mull expression driven by 1 gene
 
Mutation details: A targeting vector was designed to insert a nuclear-localized Cre recombinase gene and polyA signal followed by an FRT-flanked PGK-Neo cassette into the initiation codon of the otoferlin (Otof) locus. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice to establish the colony. Next, mutant mice were bred with FLPe-expressing mice on a C57BL/6 congenic background to remove the FRT-flanked PGK-Neo cassette. The resulting Otof-Cre mice were then bred to C57BL/6J inbred mice for approximately two generations (selecting away from the FLPe transgene). (J:151755)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Otof (mouse)
Summary of all recombinase alleles driven by Otof.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Otof Mutation:  110 strains or lines available
References
Original:  J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009;
All:  8 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory