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Pou5f1tm1.1(cre/Esr1*)Yseg
Targeted Allele Detail
Summary
Symbol: Pou5f1tm1.1(cre/Esr1*)Yseg
Name: POU domain, class 5, transcription factor 1; targeted mutation 1.1, Yoav Segal
MGI ID: MGI:5049897
Synonyms: Oct4CreERT2, Oct4-MerCreMer
Gene: Pou5f1  Location: Chr17:35816929-35821674 bp, + strand  Genetic Position: Chr17, 18.69 cM
Alliance: Pou5f1tm1.1(cre/Esr1*)Yseg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:194647
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Pou5f1tm1.1(cre/Esr1*)Yseg expression driven by 1 gene
 
Mutation details: A targeting vector containing an FRT-site flanked NEO cassette and IRES-MerCreMer sequence was inserted in the 3' UTR of Oct4/Pou5f1 locus. The construct was electroporated into C57BL/6 derived embryonic stem (ES) cells from inGenious Targeting Laboratory. Correctly targeted ES cells were injected into recipient B6(Cg)-Tyrc-2J/J or BALB/c blastocysts. The resulting chimeric animals were crossed to B6.Cg-Tg(ACTFLPe)9205Dym/J mice to remove the FRT site flanked selection cassette. (J:194647)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Pou5f1 (mouse)
Summary of all recombinase alleles driven by Pou5f1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pou5f1 Mutation:  82 strains or lines available
References
Original:  J:194647 Greder LV, et al., Analysis of endogenous Oct4 activation during induced pluripotent stem cell reprogramming using an inducible Oct4 lineage label. Stem Cells. 2012 Nov;30(11):2596-601
All:  16 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory